Huang Hai, Guo Jing, Zhang Yuxiu
School of Chemical and Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083, China.
Int J Environ Res Public Health. 2020 Dec 8;17(24):9157. doi: 10.3390/ijerph17249157.
Fully mechanized mining technology applied over a very large working face is typically utilized for coal exploitation in Northwest China and triggered two types of land subsidence above the goaf edge and center. However, the effects of mining subsidence on arbuscular mycorrhizal fungal (AMF) communities are still unknown. Here, we investigated the soil physicochemical properties and the response of AMF communities to the soil environment at the margin and center of the subsidence area of the same working face. Our results showed the soil water content, nutrient content and enzyme activity were significantly decreased with land desertification at the margin of the subsidence area but were less affected at the subsidence center. Utilizing high-throughput sequence analysis, six Glomeromycotan genera were detected. The relative abundance of Glomus and Ambispora at the margin of the subsidence area decreased, while Paraglomus and Diversispora increased. The total OTU richness was significantly correlated with moisture. Redundancy analysis showed the main environmental factors driving the changes in AMF community structure were available nitrogen, available potassium and available phosphorus. All these results indicated land cracks need to be repaired in time at subsidence edge to prevent the decline of soil fertility.
综采技术应用于超大采场，在中国西北煤炭开采中广泛使用，在采空区边缘和中心引发了两种类型的地表沉陷。然而，开采沉陷对丛枝菌根真菌（AMF）群落的影响仍不清楚。在此，我们研究了同一工作面沉陷区边缘和中心的土壤理化性质以及AMF群落对土壤环境的响应。我们的结果表明，随着沉陷区边缘土地荒漠化，土壤含水量、养分含量和酶活性显著降低，但在沉陷中心受影响较小。利用高通量序列分析，检测到6个球囊霉属。沉陷区边缘球囊霉属和双孢无梗囊霉属的相对丰度降低，而类球囊霉属和多样孢囊霉属增加。总OTU丰富度与土壤湿度显著相关。冗余分析表明，驱动AMF群落结构变化的主要环境因子是有效氮、有效钾和有效磷。所有这些结果表明，需要及时修复沉陷边缘的土地裂缝，以防止土壤肥力下降。
