Medical and Surgical Research Unit, Instituto de Investigación Sanitaria Puerta de Hierro-Segovia de Arana, Hospital Universitario Puerta de Hierro-Majadahonda, Madrid, Spain.
Medical and Surgical Research Unit, Instituto de Investigación Sanitaria Puerta de Hierro-Segovia de Arana, Hospital Universitario Puerta de Hierro-Majadahonda, Madrid, Spain.
Vet Anaesth Analg. 2021 Jan;48(1):74-81. doi: 10.1016/j.vaa.2020.04.017. Epub 2020 Oct 15.
To investigate the effect of cannabidiol (CBD) on sevoflurane minimum alveolar concentration (MAC) reduction produced by morphine in rats.
Randomized, blinded trial.
A total of 75 male Wistar Han rats weighing 276 ± 23 g (mean and standard deviation), aged 3 months.
Cannabidiol (CBD) was prepared in an ethanol-solutol-saline vehicle. Animals were randomly divided into 15 groups and given an intraperitoneal bolus of 1, 3, 5, 6.5, 7.5 or 10 mg kg of CBD alone (CBD1, CBD3, CBD5, CBD6.5, CBD7.5 and CBD10 respectively) or combined with 5 mg kg of morphine (MOR+CBD1, MOR+CBD3, MOR+CBD5, MOR+CBD6.5, MOR+CBD7.5 and MOR+CBD10). While three controls groups: MOR+saline, MOR+vehicle and vehicle were given an intraperitoneal bolus of morphine with saline, morphine with vehicle or vehicle alone respectively. The MAC was determined from alveolar gas samples at the time of tail clamp application. The MAC reduction was analyzed using a one-way ANOVA followed by Tukey's test. Additionally, Kruskal-Wallis test for non-normally-distributed data was performed. Data are presented as mean ± standard deviation. P < 0.05 RESULTS: The mean MAC was not reduced by the action of CBD administered alone, but the addition of morphine to the different doses of CBD significantly reduced the MAC. That reduction was greatest in the MOR+CBD1, MOR+CBD7.5 and MOR+CBD10 groups (29 ± 5%, 32 ± 5% and 30 ± 6% respectively), less in MOR+CBD3 and MOR+CBD6.5 groups (24 ± 3% and 26 ± 4% respectively) and least in MOR+CBD5 group (17 ± 2%). However, only the MOR+CBD5 group was statistically significantly different from MOR+CBD1, MOR+CBD7.5 and MOR+CBD10 groups.
MAC in rat was unaltered by the action of CBD alone, the reduction in MAC produced by morphine was not enhanced by the addition of CBD at the doses studied.
研究大麻二酚（CBD）对吗啡降低大鼠七氟醚肺泡最低有效浓度（MAC）的影响。
随机、双盲试验。
75 只雄性 Wistar Han 大鼠，体重 276 ± 23g（均值和标准差），3 月龄。
将大麻二酚（CBD）制成乙醇-聚乙二醇辛基苯基醚-生理盐水溶液。动物随机分为 15 组，分别给予腹腔内推注 1、3、5、6.5、7.5 或 10mg/kg 的 CBD （CBD1、CBD3、CBD5、CBD6.5、CBD7.5 和 CBD10）或联合 5mg/kg 吗啡（MOR+CBD1、MOR+CBD3、MOR+CBD5、MOR+CBD6.5、MOR+CBD7.5 和 MOR+CBD10）。同时，三个对照组（MOR+生理盐水、MOR+载体和载体）分别给予腹腔内推注吗啡+生理盐水、吗啡+载体和载体。在夹尾时，从肺泡气体样本中测定 MAC。采用单因素方差分析（ANOVA）结合 Tukey 检验分析 MAC 降低率。此外，对非正态分布数据进行了 Kruskal-Wallis 检验。数据以均数 ± 标准差表示。P<0.05。
单独使用 CBD 不会降低平均 MAC，但在不同剂量的 CBD 中加入吗啡可显著降低 MAC。在 MOR+CBD1、MOR+CBD7.5 和 MOR+CBD10 组中降低幅度最大（分别为 29 ± 5%、32 ± 5%和 30 ± 6%），在 MOR+CBD3 和 MOR+CBD6.5 组中降低幅度较小（分别为 24 ± 3%和 26 ± 4%），在 MOR+CBD5 组中降低幅度最小（17 ± 2%）。然而，只有 MOR+CBD5 组与 MOR+CBD1、MOR+CBD7.5 和 MOR+CBD10 组之间存在统计学差异。
单独使用 CBD 不会改变大鼠的 MAC，在研究剂量下，吗啡引起的 MAC 降低不会因添加 CBD 而增强。
