Lim Jolyn Jia Jia, Koh Jace, Moo Jia Rong, Villanueva Erielle Marie Fajardo, Putri Dhira Anindya, Lim Yuen Shan, Seetoh Wei Song, Mulupuri Sriya, Ng Janice Wan Zhen, Nguyen Nhi Le Uyen, Reji Rinta, Foo Herman, Zhao Margaret Xuan, Chan Tong Ling, Rodrigues Edbert Edric, Kairon Ryanjit Singh, Hee Ker Min, Chee Natasha Cassandra, Low Ann Don, Chen Zoe Hui Xin, Lim Shan Chun, Lunardi Vanessa, Fong Tuck Choy, Chua Cherlyn Xin'Er, Koh Kenny Ting Sween, Julca Irene, Delli-Ponti Riccardo, Ng Jonathan Wei Xiong, Mutwil Marek
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
College of Medicine and Veterinary Medicine, University of Edinburgh, Old College, South Bridge, Edinburgh EH8 9YL, United Kingdom.
Comput Struct Biotechnol J. 2020 Nov 20;18:3788-3795. doi: 10.1016/j.csbj.2020.11.019. eCollection 2020.
The fungi kingdom is composed of eukaryotic heterotrophs, which are responsible for balancing the ecosystem and play a major role as decomposers. They also produce a vast diversity of secondary metabolites, which have antibiotic or pharmacological properties. However, our lack of knowledge of gene function in fungi precludes us from tailoring them to our needs and tapping into their metabolic diversity. To help remedy this, we gathered genomic and gene expression data of 19 most widely-researched fungi to build an online tool, fungi.guru, which contains tools for cross-species identification of conserved pathways, functional gene modules, and gene families. We exemplify how our tool can elucidate the molecular function, biological process and cellular component of genes involved in various biological processes, by identifying a secondary metabolite pathway producing gliotoxin in , the catabolic pathway of cellulose in and the conserved DNA replication pathway in and . The tool is available at www.fungi.guru.
真菌界由真核异养生物组成，它们负责平衡生态系统，并作为分解者发挥主要作用。它们还产生种类繁多的次生代谢产物，这些次生代谢产物具有抗生素或药理特性。然而，我们对真菌基因功能的了解不足，这使我们无法根据自身需求对它们进行改造，也无法利用它们的代谢多样性。为了帮助解决这一问题，我们收集了19种研究最广泛的真菌的基因组和基因表达数据，构建了一个在线工具fungi.guru，它包含用于跨物种鉴定保守途径、功能基因模块和基因家族的工具。我们通过鉴定烟曲霉中产生胶霉毒素的次生代谢途径、里氏木霉中纤维素的分解代谢途径以及酿酒酵母和白色念珠菌中保守的DNA复制途径，举例说明了我们的工具如何阐明参与各种生物过程的基因的分子功能、生物学过程和细胞成分。该工具可在www.fungi.guru上获取。
