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木质纤维素降解:参与木质纤维素降解的真菌及真菌酶概述

Lignocellulose degradation: An overview of fungi and fungal enzymes involved in lignocellulose degradation.

作者信息

Andlar Martina, Rezić Tonči, Marđetko Nenad, Kracher Daniel, Ludwig Roland, Šantek Božidar

机构信息

Department of Biochemical Engineering Faculty of Food Technology and Biotechnology University of Zagreb Zagreb Croatia.

Department of Food Sciences and Technology University of Natural Resources and Life Sciences Vienna Austria.

出版信息

Eng Life Sci. 2018 Jun 27;18(11):768-778. doi: 10.1002/elsc.201800039. eCollection 2018 Nov.

Abstract

This review aims to present current knowledge of the fungi involved in lignocellulose degradation with an overview of the various classes of lignocellulose-acting enzymes engaged in the pretreatment and saccharification step. Fungi have numerous applications and biotechnological potential for various industries including chemicals, fuel, pulp, and paper. The capability of fungi to degrade lignocellulose containing raw materials is due to their highly effective enzymatic system. Along with the hydrolytic enzymes consisting of cellulases and hemicellulases, responsible for polysaccharide degradation, they have a unique nonenzymatic oxidative system which together with ligninolytic enzymes is responsible for lignin modification and degradation. An overview of the enzymes classification is given by the Carbohydrate-Active enZymes (CAZy) database as the major database for the identification of the lignocellulolytic enzymes by their amino acid sequence similarity. Finally, the recently discovered novel class of recalcitrant polysaccharide degraders-lytic polysaccharide monooxygenases (LPMOs) are presented, because of these enzymes importance in the cellulose degradation process.

摘要

本综述旨在介绍目前关于参与木质纤维素降解的真菌的知识,并概述参与预处理和糖化步骤的各类木质纤维素作用酶。真菌在包括化工、燃料、纸浆和造纸等多个行业有众多应用和生物技术潜力。真菌降解含木质纤维素原料的能力归因于其高效的酶系统。除了由负责多糖降解的纤维素酶和半纤维素酶组成的水解酶外,它们还有独特的非酶氧化系统,该系统与木质素分解酶一起负责木质素的修饰和降解。碳水化合物活性酶(CAZy)数据库给出了酶分类的概述,该数据库是通过氨基酸序列相似性鉴定木质纤维素分解酶的主要数据库。最后,介绍了最近发现的新型难降解多糖降解酶——裂解多糖单加氧酶(LPMO),因为这些酶在纤维素降解过程中具有重要作用。

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