Department of Molecular Biology and Genetics, Gebze Technical University, Gebze-Kocaeli, 41400, Turkey.
Department of Biology, Illinois Institute of Technology, Chicago, IL.
Lett Appl Microbiol. 2021 Apr;72(4):484-494. doi: 10.1111/lam.13440. Epub 2021 Jan 4.
Enhancement of the desulfurization activities of Paenibacillus strains 32O-W and 32O-Y were investigated using dibenzothiophene (DBT) and DBT sulfone (DBTS) as sources of sulphur in growth experiments. Strains 32O-W, 32O-Y and their co-culture (32O-W plus 32O-Y), and Vitreoscilla hemoglobin (VHb) expressing recombinant strain 32O-Yvgb and its co-culture with strain 32O-W were grown at varying concentrations (0·1-2 mmol l ) of DBT or DBTS for 96 h, and desulfurization measured by production of 2-hydroxybiphenyl (2-HBP) and disappearance of DBT or DBTS. Of the four cultures grown with DBT as sulphur source, the best growth occurred for the 32O-Yvgb plus 32O-W co-culture at 0·1 and 0·5 mmol l DBT. Although the presence of vgb provided no consistent advantage regarding growth on DBTS, strain 32O-W, as predicted by previous work, was shown to contain a partial 4S desulfurization pathway allowing it to metabolize this 4S pathway intermediate.
采用二苯并噻吩（DBT）和二苯并噻吩砜（DBTS）作为生长实验中的硫源，研究了芽孢杆菌菌株 32O-W 和 32O-Y 的脱硫活性。在 96 h 内，用不同浓度（0·1-2 mmol·l）的 DBT 或 DBTS 培养菌株 32O-W、32O-Y 及其共培养物（32O-W 加 32O-Y）、表达 Vitreoscilla 血红蛋白（VHb）的重组菌株 32O-Yvgb 及其与菌株 32O-W 的共培养物，并通过生成 2-羟基联苯（2-HBP）和 DBT 或 DBTS 的消失来测量脱硫。在以 DBT 作为硫源的四种培养物中，32O-Yvgb 加 32O-W 共培养物在 0·1 和 0·5 mmol·l DBT 下生长最好。尽管 vgb 的存在在 DBTS 上的生长没有提供一致的优势，但正如先前的工作所预测的那样，菌株 32O-W 被证明含有部分 4S 脱硫途径，使其能够代谢这种 4S 途径中间体。
