Bagchi Asheemita, Srivastava Preeti
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, India.
Front Bioeng Biotechnol. 2024 Oct 28;12:1482270. doi: 10.3389/fbioe.2024.1482270. eCollection 2024.
Sulfur, an abundant component of crude oil, causes severe damage to the environment, poses risks to human health, and poisons the catalysts used in combustion engines. Hydrodesulfurization, the conventionally used method, is not sufficient to remove thiophenes like dibenzothiophene (DBT) and other aromatic heterocyclic compounds. The push for "ultra-clean" fuels, with sulfur content less than 15 ppm, drives the need for deep desulfurization. Thus, in conjunction with hydrodesulfurization, efficient and eco-friendly methods of deep desulfurization, like biodesulfurization, are desirable. In biodesulfurization, naturally desulfurizing microorganisms are used, with genetic engineering and biotechnology, to reduce the sulfur content of crude oil to below 15 ppm. In this review, we describe genetic and metabolic engineering approaches reported to date to develop more efficient methods to carry out biodesulfurization, making it a practically applicable reality.
硫是原油中的一种丰富成分，会对环境造成严重破坏，对人类健康构成风险，并使内燃机中使用的催化剂中毒。传统使用的加氢脱硫方法不足以去除二苯并噻吩（DBT）等噻吩和其他芳香族杂环化合物。对硫含量低于15 ppm的“超清洁”燃料的需求推动了深度脱硫的必要性。因此，与加氢脱硫相结合，高效且环保的深度脱硫方法，如生物脱硫，是很有必要的。在生物脱硫中，利用天然脱硫微生物，通过基因工程和生物技术，将原油的硫含量降低至15 ppm以下。在本综述中，我们描述了迄今为止报道的遗传和代谢工程方法，以开发更有效的生物脱硫方法，使其成为实际可行的现实。
