Wu Shuo, Zhang Yao, Zhang Yan, Chen Lizhan, Xu Xi, Dang Yinli, Ti Xinyu
Department of Pulmonary Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Diagnosis and Treatment Center, Wuxi, China.
Pharmazie. 2020 Oct 1;75(10):512-515. doi: 10.1691/ph.2020.0558.
An increasing number of studies have demonstrated that phillygenin (PG) exerts anti-oxidant, anti-inflammatory and anti-cancer activities. However, the effects of PG on the proliferation and invasion in non-small cell lung cancer (NSCLC) cells have not been clarified. In this study, MTT assay and flow cytometry were conducted to investigate the effect of PG on proliferation and apoptosis of NSCLC cells , respectively. A xenograft model of A549 cell was established in nude mice to validate the findings. Western blot were performed to measure the expression of molecules involved in AMPK/ERK/NF-κB pathway. Results suggested that PG (50 or 100 μM) was significantly cytotoxic to A549 cells and SPC-A1 cells . PG treatment also inhibited the tumor growth of NSCLC cell mouse xenografts . These anti-proliferative and pro-apoptosis effects of PG were found to be regulated by the AMPK/ERK/NF-κB pathway. Consequently, PG suppressed proliferation and induced cell apoptosis in NSCLC cells. In conclusions, PG regulates AMPK/ERK/NF-κB axis in NSCLC cells, thereby inhibiting the proliferation and promoting the apoptosis of NSCLC cells.
越来越多的研究表明，连翘酯苷（PG）具有抗氧化、抗炎和抗癌活性。然而，PG对非小细胞肺癌（NSCLC）细胞增殖和侵袭的影响尚未阐明。在本研究中，分别进行MTT试验和流式细胞术以研究PG对NSCLC细胞增殖和凋亡的影响。在裸鼠中建立A549细胞异种移植模型以验证研究结果。进行蛋白质免疫印迹法以检测AMPK/ERK/NF-κB途径中相关分子的表达。结果表明，PG（50或100μM）对A549细胞和SPC-A1细胞具有显著的细胞毒性。PG处理还抑制了NSCLC细胞小鼠异种移植瘤的生长。发现PG的这些抗增殖和促凋亡作用受AMPK/ERK/NF-κB途径调节。因此，PG抑制NSCLC细胞的增殖并诱导细胞凋亡。总之，PG调节NSCLC细胞中的AMPK/ERK/NF-κB轴，从而抑制NSCLC细胞的增殖并促进其凋亡。
