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蜂胶、臭氧和光动力疗法用于根管消毒对树脂与牙根牙本质粘结强度的影响。

Influence of Propolis, Ozone and Photodynamic therapy in root canal disinfection on resin bond strength to radicular dentin.

作者信息

Almadi Khalid, Alkahtany Mazen, Alamam Yousef, Alaql Fahad, Alaqil Abdulaziz, Almutairi Meshari, Thafrah Saud Mohammed Bin

机构信息

Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.

BDS King Saud University, Riyadh, Saudi Arabia.

出版信息

Photodiagnosis Photodyn Ther. 2021 Mar;33:102131. doi: 10.1016/j.pdpdt.2020.102131. Epub 2020 Dec 8.

Abstract

AIM

The aim of the present study was to assess post space disinfection using different irrigants and their effect on radicular dentin bond to SERC.

MATERIALS AND METHODS

Forty single rooted mandibular 2nd premolars were collected and disinfected. All samples were decoronated maintaining root length of 12 mm and embedded vertically in acrylic resin. Shaping of the canal was performed using reciprocating system. Canals were dried using paper points and obturated with gutta percha via lateral condensation. Peso-reamers were used to create post space up to 10 mm. The post space was irrigated with 1% NaOCl before being randomly allocated into four groups (n = 10) according to different methods of canal disinfection. Samples in group 1 were subjected to photoactivated disinfection (PAD), group 2 irrigated with ethanol based propolis group 3 disinfected using ozone and group 4 irrigated with 2.25 % NaOCl with 17 % EDTA and ECL. GFP was fitted using SERC. Each sample was cut into 1 mm from coronal, middle and apical and subjected to pushout bond strength (PBS) via universal testing machine. Fracture analysis were confirmed by stereomicroscope at 40x magnification. For comparison of means, Analysis of variance (ANOVA) and Tukey multiple comparison test was used maintaining the level of significance at p <  0.05.

RESULTS

The highest PBS at all three levels were found in group 4, canal disinfected with 2.5 % NaOCl with 17 % EDTA and ECYL. Whereas, the lowest PBS was observed in group 1. Inter group comparison, prosthetic space disinfected with propolis extract showed no significant difference to group 4 canal disinfected with 2.5 % NaOCl with 17 % EDTA and ECYL at all three levels (p > 0.05). Intra group comparison in all experimental groups showed no significant difference was noted in coronal and middle thirds of group 1, group 2 and group 3 respectively (p > 0.05).

CONCLUSION

2.5 % NaOCl with EDTA and ECL treatment (group 4) remains the gold standard when used as a final canal irrigant. Propolis can be used as potential irrigant for canal disinfectant provided if the composition becomes standardized.

摘要

目的

本研究旨在评估使用不同冲洗剂进行桩道消毒及其对根管牙本质与SERC粘结的影响。

材料与方法

收集40颗单根下颌第二前磨牙并进行消毒。所有样本均截冠,保留12毫米的牙根长度,并垂直嵌入丙烯酸树脂中。使用往复系统进行根管预备。用纸尖干燥根管,通过侧向加压法用牙胶进行充填。使用Peso扩孔钻制备深度达10毫米的桩道。在随机分为四组(n = 10)之前,先用1%次氯酸钠冲洗桩道,根据不同的根管消毒方法分组。第1组样本接受光活化消毒(PAD),第2组用乙醇基蜂胶冲洗,第3组用臭氧消毒,第4组用2.25%次氯酸钠与17%乙二胺四乙酸(EDTA)及乙二胺氯己定(ECL)冲洗。使用SERC粘结绿色荧光蛋白(GFP)。将每个样本从冠方、中部和根尖部切成1毫米厚的薄片,通过万能试验机进行推出粘结强度(PBS)测试。通过40倍放大的体视显微镜确认断裂分析结果。为比较均值,采用方差分析(ANOVA)和Tukey多重比较检验,显著性水平设定为p < 0.05。

结果

在所有三个层面,第4组(用2.5%次氯酸钠与17% EDTA及ECL冲洗根管)的PBS最高。而第1组的PBS最低。组间比较,用蜂胶提取物消毒的修复空间与第4组(用2.5%次氯酸钠与17% EDTA及ECL冲洗根管)在所有三个层面均无显著差异(p > 0.05)。所有实验组的组内比较显示,第1组、第2组和第3组在冠方和中部三分之一处分别无显著差异(p > 0.05)。

结论

当用作最终根管冲洗剂时,2.5%次氯酸钠与EDTA及ECL联合处理(第4组)仍是金标准。如果蜂胶成分标准化,可作为根管消毒剂的潜在冲洗剂。

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