Institute of Biotechnology, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road, Caugiay Distr., 100000, Hanoi, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Viet Nam.
Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia.
Chemosphere. 2021 Feb;265:129167. doi: 10.1016/j.chemosphere.2020.129167. Epub 2020 Dec 2.
Actinoplanes sp. A1094 strain had been selected for its high production of acarbose from 20 different strains of Actinoplanes sp. can be found in wild. The content for glucosidase inhibitor of acarbose concentration was recorded at 1.12 g/L. The conducted analysis of 16S rRNA sequence of Actinoplanes sp. A1094 showed 99% similar identity to the corresponding sequence of Actinoplanes hulinensis. Acarbose was purified from Actinoplanes hulinensis 1094 with a yield of 8.48%, purity of 98% and further identified by LC/MS and NMR methods (CHNO; m/z: 645.6 g/mol). The purified acarbose was used to evaluate the hypoglycemia in streptozotocin (STZ)-induced diabetic mice model. The purified acarbose reduced postprandial blood glucose level in comparison with Glucobay® as medication for control type 2 diabetes in a combination therapy. Notably, the outcomes of native acarbose on fasting blood glucose levels in mice resemble akin to the commercial product and the acarbose accumulating fermentation and metabolic engineering from the cell gene in which would reduce in production cost. Therefore, acarbose from Actinoplanes hulinensis 1094 could be potentially used to make products for the treatment of type II diabetes.
放线菌 sp. A1094 菌株因其能够从 20 种不同的放线菌 sp. 中高产阿卡波糖而被选中，可以在野外找到。阿卡波糖的葡萄糖苷酶抑制剂浓度含量记录为 1.12 g/L。对放线菌 sp. A1094 的 16S rRNA 序列进行分析，显示其与放线菌 hulinensis 的相应序列具有 99%的相似性。阿卡波糖从放线菌 hulinensis 1094 中纯化，产率为 8.48%，纯度为 98%，并通过 LC/MS 和 NMR 方法进一步鉴定（CHNO；m/z：645.6 g/mol）。纯化的阿卡波糖用于评估阿卡波糖对链脲佐菌素（STZ）诱导的糖尿病小鼠模型的降血糖作用。与作为治疗 2 型糖尿病的药物的 Glucobay®相比，纯化的阿卡波糖降低了餐后血糖水平。值得注意的是，天然阿卡波糖对小鼠空腹血糖水平的影响与商业产品相似，阿卡波糖的积累发酵和代谢工程可以降低生产成本。因此，从放线菌 hulinensis 1094 中提取的阿卡波糖可能可用于生产治疗 II 型糖尿病的产品。
