Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Mengniu Hi-tech Dairy Product Beijing Co., Ltd., Beijing 101100, China P.R.
Benef Microbes. 2021 Feb 24;12(1):31-42. doi: 10.3920/BM2020.0023. Epub 2020 Dec 14.
Probiotics have been reported to be associated with the alleviation of constipation. The aim of this study was to detect and determine the effect of subsp. MN-Gup (MN-Gup) on the alleviation of constipation in BALB/c mice and humans, and to elucidate the mechanisms underlying its effect by measuring changes in the concentration of short-chain fatty acids and the composition of microbes in human faeces. BALB/c mice were given MN-Gup by gavage for 14 days. On the 8 day of this treatment, constipation was induced by the application of diphenoxylate via gavage. The results showed that MN-Gup significantly decreased the first black stool defecation time, and significantly increased black faecal wet weight, black faecal number and the gastric-intestinal transit rate (<0.05), thereby relieving constipation. In humans, a randomised, double-blind, placebo-controlled trial was performed to investigate the effect of MN-Gup in adults with functional constipation. After 4 weeks of intervention with placebo or MN-Gup yogurt, constipation-related symptoms (including defecation frequency, stool consistency, straining and incomplete feeling during defecation) in the constipated subjects were significantly improved in the two groups, but not different between the groups at the end of the intervention. The concentration of acetate increased significantly in the MN-Gup group compared to the placebo group and before ingestion. Significant changes in the composition of gut microbiota were found after intake of MN-Gup yogurt when compared to placebo. The relative abundances of acetate-producing and were significantly increased after intake of MN-Gup yogurt. These results showed that MN-Gup could relieve constipation related to increased acetate-producing and .
益生菌已被报道与缓解便秘有关。本研究旨在检测和确定 subsp. MN-Gup (MN-Gup) 对 BALB/c 小鼠和人类便秘缓解的影响，并通过测量短链脂肪酸浓度和粪便微生物组成的变化来阐明其作用机制。BALB/c 小鼠通过灌胃给予 MN-Gup 14 天。在治疗的第 8 天，通过灌胃给予地芬诺酯诱导便秘。结果表明，MN-Gup 可显著缩短首次黑便排便时间，显著增加黑便湿重、黑便数和胃-肠传输率(<0.05)，从而缓解便秘。在人类中，进行了一项随机、双盲、安慰剂对照试验，以研究 MN-Gup 对功能性便秘成人的影响。在安慰剂或 MN-Gup 酸奶干预 4 周后，便秘受试者的便秘相关症状(包括排便频率、粪便稠度、排便时用力和排不尽感)在两组均显著改善，但干预结束时两组之间无差异。与安慰剂组相比，MN-Gup 组乙酸浓度显著升高，且高于摄入前水平。与安慰剂相比，摄入 MN-Gup 酸奶后肠道微生物群组成发生了显著变化。摄入 MN-Gup 酸奶后，产乙酸菌和产丁酸菌的相对丰度显著增加。这些结果表明，MN-Gup 可缓解与产乙酸菌和产丁酸菌增加相关的便秘。
