Department of Dental Pharmacology, School of Dentistry, Pusan National University, Yangsan-si 50612, Republic of Korea.
Periodontal Disease Signaling Network Research Center (MRC), School of Dentistry, Pusan National University, Yangsan-si 50612, Republic of Korea.
Am J Chin Med. 2020;48(8):1875-1893. doi: 10.1142/S0192415X20500949. Epub 2020 Dec 10.
Inflammation regulation is essential for maintaining healthy functions and normal homeostasis of the body. () is a gram-negative anaerobic bacterium and a major pathogen that causes oral inflammation and other systemic inflammations. This study aims to examine the anti-inflammatory effects of Ledeb root extracts (APL-ME) in LPS-induced RAW 264.7 cells and find anti-inflammatory effect compounds of APL-ME. The anti-inflammatory effects of APL-ME were evaluated anti-oxidant activity, cell viability, nitrite concentration, pro-inflammatory cytokines (interleukin-1[Formula: see text], interleukin-6, tumor necrosis factor (TNF)-[Formula: see text], and anti-inflammatory cytokine (interleukin-10 (IL-10)). Also, Inflammation related genes and proteins, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), expression were decreased by APL-ME and mitogen-activated protein kinase (MAPK) signaling proteins expression was regulated by APL-ME. Liquid chromatography-mass spectrometer (LC/MS)-MS analysis results indicated that several components were detected in APL-ME. Our study indicated that APL-ME suppressed nitrite concentrations, pro-inflammatory cytokines such as IL-1[Formula: see text], IL-6 and TNF-[Formula: see text] in LPS induced RAW 264.7 cells. However, IL-10 expression was increased by ALP-ME. In addition, protein expressions of COX-2 and iNOS were inhibited APL-ME extracts dose-dependently. According to these results, APL-ME has anti-inflammatory effects in LPS induced RAW 264.7 cells.
炎症调节对于维持身体的健康功能和正常内稳态至关重要。（牙龈卟啉单胞菌）是一种革兰氏阴性厌氧菌，也是引起口腔炎症和其他全身炎症的主要病原体。本研究旨在研究 Ledeb 根提取物（APL-ME）在 LPS 诱导的 RAW 264.7 细胞中的抗炎作用，并寻找 APL-ME 的抗炎作用化合物。通过抗氧化活性、细胞活力、亚硝酸盐浓度、促炎细胞因子（白细胞介素-1[Formula: see text]、白细胞介素-6、肿瘤坏死因子（TNF）-[Formula: see text]和抗炎细胞因子（白细胞介素-10（IL-10））来评估 APL-ME 的抗炎作用。此外，APL-ME 还降低了与炎症相关的基因和蛋白质（环氧合酶（COX）-2、诱导型一氧化氮合酶（iNOS））的表达，并调节丝裂原激活蛋白激酶（MAPK）信号蛋白的表达。液质联用仪（LC/MS）-MS 分析结果表明，APL-ME 中检测到几种成分。我们的研究表明，APL-ME 抑制了 LPS 诱导的 RAW 264.7 细胞中硝酸盐浓度、促炎细胞因子如白细胞介素-1[Formula: see text]、白细胞介素-6 和 TNF-[Formula: see text]的表达。然而，APL-ME 增加了 IL-10 的表达。此外，APL-ME 提取物还抑制了 COX-2 和 iNOS 的蛋白表达，呈剂量依赖性。根据这些结果，APL-ME 对 LPS 诱导的 RAW 264.7 细胞具有抗炎作用。
