Cancer Preventive Material Development Research Center, College of Oriental medicine, Kyunghee University, Seoul, Republic of Korea.
Immunol Invest. 2010 Jan;39(2):159-70. doi: 10.3109/08820130903501790.
Herbal medicines including Agrimonia pilosa Ledeb. (APL) have been traditionally used to treat inflammations including allergic disease as valuable medicinal properties. To investigate the attenuating ability of APL on inflammation, the NO release and ROS production, which play a key role in inflammatory and immune responses, was first tested using in vitro assay. The 80% ethanol extract of APL showed a significant activity to inhibit NO release and ROS production. In additional extracts from 80% ethanol extract of APL, n-butanol (BuOH) extract displayed the most potent anti-inflammatory effects based on in vitro assay. The extract also significantly reduced nitric oxide in lipopolysaccharide-activated RAW 264.7 macrophage cells (p < 0.05), and suppressed the nitric oxide synthase (iNOS) expression, whereas the extract showed no inhibitory effect on cyclooxygenase-2 (COX-2) expression, suggesting that the BuOH extract of APL could reduce the NO production through suppression of iNOS, but not COX-2. The BuOH extract also showed a significant effect in a carrageenan-induced rat paw edema in vivo model, consistent with our in vitro results. Our findings suggest that the BuOH extract of APL shows a potential anti-inflammatory activity, substantiating its traditional use in medicine.
包括仙鹤草（APL）在内的草药已被传统用于治疗炎症，包括作为有价值药用特性的过敏性疾病。为了研究 APL 对炎症的减弱能力，首先使用体外测定法测试了在炎症和免疫反应中起关键作用的 NO 释放和 ROS 产生。APL 的 80％乙醇提取物显示出抑制 NO 释放和 ROS 产生的显著活性。在 APL 的 80％乙醇提取物的其他提取物中，基于体外测定，正丁醇（BuOH）提取物显示出最强的抗炎作用。该提取物还显著减少了脂多糖激活的 RAW 264.7 巨噬细胞细胞中的一氧化氮（p <0.05），并抑制了一氧化氮合酶（iNOS）的表达，而该提取物对环加氧酶-2（COX-2）的表达没有抑制作用，表明 BuOH 提取物可通过抑制 iNOS 而不是 COX-2 来减少 NO 的产生。BuOH 提取物在体内角叉菜胶诱导的大鼠足肿胀模型中也显示出显著效果，与我们的体外结果一致。我们的研究结果表明，APL 的 BuOH 提取物具有潜在的抗炎活性，证实了其在医学中的传统用途。
