School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China.
Chin J Nat Med. 2020 Nov;18(11):872-880. doi: 10.1016/S1875-5364(20)60030-9.
The depressant-like effects of albiflorin (AF) were studied on stressed chronic restraint stress (CRS) rats. Experimental rats were subjected to immobilization stress for a daily 6 h-restraining in a plastic restrainer for continuous 21 d and were treated with 30 or 15 mg·kg of AF for 21 d. Control rats were maintained in completely non stressed conditions. Behavioral tests and biochemical analysis were applied to investigating a regulatory mechanism of anti-stress of AF. Treatment with AF significantly restored the depressant-like behaviors. Besides, AF increased the levels of 5-hydroxytryptophan (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), noradrenaline (NE) and dopamine (DA) in the hippocampus and increased the level of brain-derived neurotrophic factor (BDNF) in serum and protein expression in hippocampus. In addition, AF decreased the levels of hypothalamo-pituitary-adrenal (HPA) cascade, reduced the level of NO and cGMP in serum and inhibited the overexpression of 5-HTR mRNA and protein expression. Taken together, AF can modulate the NO-mediated network pathway in the hippocampus against stress-induced depressive-like behaviors. These physiological and behavioral changes allow rats to avoid potential deleterious effects of stress that may result from chronically elevated levels of glucocorticosteroids over days.
白芍总苷（AF）对慢性束缚应激（CRS）大鼠的抑制作用进行了研究。实验大鼠每天在塑料固定器中固定 6 小时，连续 21 天，并给予 30 或 15 mg·kg 的 AF 治疗 21 天。对照大鼠保持在完全非应激状态。应用行为测试和生化分析来研究 AF 抗应激的调节机制。AF 治疗显著恢复了抑郁样行为。此外，AF 增加了海马中 5-羟色氨酸（5-HT）、5-羟吲哚乙酸（5-HIAA）、去甲肾上腺素（NE）和多巴胺（DA）的水平，增加了血清中脑源性神经营养因子（BDNF）的水平和海马中的蛋白表达。此外，AF 降低了下丘脑-垂体-肾上腺（HPA）级联反应的水平，降低了血清中 NO 和 cGMP 的水平，并抑制了 5-HTR mRNA 和蛋白表达的过度表达。总之，AF 可以调节海马中 NO 介导的网络通路，对抗应激引起的抑郁样行为。这些生理和行为的变化使大鼠能够避免由于糖皮质激素水平长期升高而导致的潜在有害影响。
