Zhu Ying-Li, Wang Lin-Yuan, Wang Cheng-Long, Zhao Dan-Ping, Wang Sha, Fei Wen-Ting, Zhang Jian-Jun
Beijing University of Chinese Medicine, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4240-4246. doi: 10.4268/cjcmm20162225.
This paper aimed to investigate the antidepressant-like effects and the mechanism of the albiflorin, paeoniflorin on rats with chronic restraint stress model. Fluoxetine and Xiaoyao group served as the positive control, body weight, sucrose preference test and the open field behavioral experiment were measured, the levels of nitric oxide (NO) in hippocampus were detected by Greg colorimetric method. Furthermore, the levels of brain derived neurotrophic factor (BDNF) in hippocampus were detected by ELISA. Finally, the expressions of nNOS mRNA in hippocampus detected by RT-qPCR, the protein levels of nNOS and BDNF in hippocampus were detected by Western blot. Compared with the model group,the pass counts of paeoniflorin group(30 mg•kg⁻¹) and albiflorin group(30 mg•kg⁻¹) were obviously increased(P<0.05,P<0.01). Furthermore, compared with the model group,the levels of NO in paeoniflorin groups(30 mg•kg⁻¹ and 15 mg•kg⁻¹) and albiflorin groups(30 mg•kg⁻¹ and 15 mg•kg⁻¹) were all significantly decreased(P<0.05,P<0.01; P<0.05, P<0.05). BDNF levels of paeoniflorin group(30 mg•kg⁻¹) and albiflorin group(30 mg•kg⁻¹) were obviously increased(P<0.05). Finally, compared with the model group, the expressions of nNOS mRNA of paeoniflorin groups(30 mg•kg⁻¹ and 15 mg•kg⁻¹) (P<0.01, P<0.01) and albiflorin groups(30 mg•kg⁻¹) were significantly decreased(P<0.05). Compared with the model group, the protein exprsssions of BDNF of paeoniflorin group(30 mg•kg⁻¹) and albiflorin group(30 mg•kg⁻¹) were obviously increased(P<0.05). Albiflorin and paeoniflorin have the effects of smooth the liver and dispel stagnation, the mechanism has the relevant with adjusting and controlling the expression of NO and BDNF in hippocampus.
本文旨在研究芍药苷、芍药内酯苷对慢性束缚应激模型大鼠的抗抑郁样作用及其机制。氟西汀组和逍遥散组作为阳性对照，测定大鼠体重、蔗糖偏好试验和旷场行为实验，采用Griess比色法检测海马组织中一氧化氮（NO）水平。此外，采用酶联免疫吸附测定法（ELISA）检测海马组织中脑源性神经营养因子（BDNF）水平。最后，采用实时荧光定量聚合酶链反应（RT-qPCR）检测海马组织中nNOS mRNA的表达，采用蛋白质免疫印迹法（Western blot）检测海马组织中nNOS和BDNF的蛋白水平。与模型组相比，芍药苷组（30 mg•kg⁻¹）和芍药内酯苷组（30 mg•kg⁻¹）的穿越次数明显增加（P<0.05，P<0.01）。此外，与模型组相比，芍药苷组（30 mg•kg⁻¹和15 mg•kg⁻¹）和芍药内酯苷组（30 mg•kg⁻¹和15 mg•kg⁻¹）的NO水平均显著降低（P<0.05，P<0.01；P<0.05，P<0.05）。芍药苷组（30 mg•kg⁻¹）和芍药内酯苷组（30 mg•kg⁻¹）的BDNF水平明显升高（P<0.05）。最后，与模型组相比，芍药苷组（30 mg•kg⁻¹和15 mg•kg⁻¹）（P<0.01，P<0.01）和芍药内酯苷组（30 mg•kg⁻¹）的nNOS mRNA表达显著降低（P<0.05）。与模型组相比，芍药苷组（30 mg•kg⁻¹）和芍药内酯苷组（30 mg•kg⁻¹）的BDNF蛋白表达明显升高（P<0.05）。芍药内酯苷和芍药苷具有疏肝解郁作用，其机制与调控海马组织中NO和BDNF的表达有关。
