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伊朗亚兹德省骆驼(单峰驼)中无形体的分子检测。

Molecular detection of Anaplasma platys among camels (Camelus dromedarius) in Yazd, Iran.

机构信息

Pathobiology Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran; Pathobiology Department, Shahmirzad School of Veterinary Medicine, Semnan University, Semnan, Iran.

Pathobiology Department, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.

出版信息

Vet Parasitol Reg Stud Reports. 2020 Dec;22:100462. doi: 10.1016/j.vprsr.2020.100462. Epub 2020 Sep 8.

DOI:10.1016/j.vprsr.2020.100462
PMID:33308747
Abstract

Anaplasma platys is a tick-borne bacterium that infects platelets. In this study, A. platys bacteria were investigated in 60 blood samples of male camels (Camelus dromedarius) in Yazd, Iran by molecular examination. Anaplasma platys 16S rRNA (ribosomal ribonucleic acid) gene was detected in 2 samples out of 60 (3.33%) by molecular technique, then the blood films of positive samples were observed by a light microscope and A. platys inclusions were observed in the platelets of both infected cases. The PCR (polymerase chain reaction) products were sequenced to confirm the identification of the bacterial species. The results showed the presence of A. platys among camels in Iran.

摘要

卵形无浆体是一种感染血小板的蜱传细菌。在这项研究中,通过分子检测,对伊朗亚兹德的 60 份雄性骆驼(单峰骆驼)血液样本中的卵形无浆体细菌进行了研究。分子技术检测到 60 份样本中有 2 份(3.33%)存在卵形无浆体 16S rRNA(核糖体核糖核酸)基因,然后用光学显微镜观察阳性样本的血片,在这两种感染病例的血小板中观察到卵形无浆体包涵体。对 PCR(聚合酶链反应)产物进行测序以确认细菌种的鉴定。结果表明,伊朗的骆驼中存在卵形无浆体。

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