Ait Lbacha Hicham, Zouagui Zaid, Alali Said, Rhalem Abdelkbir, Petit Elisabeth, Ducrotoy Marie Julie, Boulouis Henri-Jean, Maillard Renaud
Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco.
Département de médecine, chirurgie et reproduction, Madinat Al Irfane, Rabat Instituts, IAV Hassan II, BP 6202, Rabat, 10101, Morocco.
Infect Dis Poverty. 2017 Feb 5;6(1):1. doi: 10.1186/s40249-016-0216-8.
There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR.
Camels (n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL. Anaplasmataceae strains were characterised by sequence analysis of the gene groEL.
A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to "Candidatus Anaplasma camelii". Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys.
This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius.
This study is not a clinical trial and therefore a trial registration number does not apply.
由于骆驼无形体病最近在这种储存宿主物种中出现，并因其人畜共患病潜力而受到关注，人们对它的兴趣与日俱增。因此，骆驼无形体病的流行病学仍知之甚少，主要是因为骆驼属于边缘化的贫困群体，且经常是游牧群体，他们的利益在很大程度上被忽视。大多数关于骆驼无形体病的研究都依赖显微镜检查和血清学进行诊断，只有在突尼斯、沙特阿拉伯和中国进行的三项研究使用了分子诊断方法。本研究使用聚合酶链反应（PCR）对摩洛哥单峰驼储存宿主中传播的无形体科菌株进行了特征分析。
从摩洛哥南部代表不同农业生态区域的6个地区随机抽取了106头骆驼。采集全血并使用针对groEL基因的PCR方法进行筛查。通过对groEL基因进行序列分析来鉴定无形体科菌株。
在筛查的骆驼中，共有39.62%（42/106）对无形体科物种呈阳性。对五个阳性测序样本进行的GenBank BLAST分析显示，所有菌株与“骆驼嗜性无形体（Candidatus Anaplasma camelii）”的相似度均为100%。对groEL基因比对片段（650 bp）的系统发育研究和遗传特征分析证实，其与犬嗜吞噬细胞无形体（A. platys）高度相似。
本研究表明，在摩洛哥存在一种以前未被鉴定的无形体属物种，该物种在基因上与引起犬无形体病的病原体接近，但据认为其主要储存宿主是单峰驼。
本研究不是临床试验，因此不需要试验注册号。
