Laboratorio de Inmunoparasitología (LAINPA), Flacultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118 (1900), La Plata, Argentina.
Laboratorio de Inmunoparasitología (LAINPA), Flacultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118 (1900), La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina.
Vet Parasitol Reg Stud Reports. 2020 Dec;22:100473. doi: 10.1016/j.vprsr.2020.100473. Epub 2020 Sep 20.
Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.
猪隐孢子虫病由不同的隐孢子虫种或基因型引起，猪特异性种包括猪隐孢子虫（C. suis）和猪隐孢子虫（C. scrofarum）。有关南美的猪隐孢子虫感染的信息很少。总共从 13 个阿根廷集约化猪场的 1、2、3 和 4 周龄仔猪（每组 130 只）中获得了 520 份单独的粪便样本。使用显微镜和分子技术结合诊断隐孢子虫物种。对显微镜下有隐孢子虫卵囊的样本进行种属和种特异性巢式 PCR 检测 18S rRNA 基因片段，并进行测序。微观分析在 11/13（85%）个农场的 47/520（9%）粪便样本中检测到隐孢子虫卵囊，农场感染率在 0%至 17.5%之间。隐孢子虫卵囊的存在与腹泻有关。显微镜阳性样本的比例与仔猪年龄无关。共有 15/47（卵囊结构相容样本的 32%）通过种属和种特异性巢式 PCR 呈阳性。种特异性 PCR 和测序显示，3、8 和 4 个样本中分别存在 C. suis、C. scrofarum 和两种物种。各特定 PCR 阳性样本的比例在年龄组之间相似，4 周龄仔猪中 C. suis 的比例略高。分子工具的使用证实了阿根廷猪的 C. suis 和 C. scrofarum 感染。隐孢子虫病在阿根廷主要养猪区广泛分布，农场内感染率较低至中等。
