CD68+ 巨噬细胞与 CD8+ T 细胞比值与乙型肝炎病毒（HBV）相关肝细胞癌的临床结局相关。

The CD68+ macrophages to CD8+ T-cell ratio is associated with clinical outcomes in hepatitis B virus (HBV)-related hepatocellular carcinoma.

机构信息

Department of Hepatic Surgery I (Ward I), Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai, China.

Genecast Biotechnology Co., Ltd, Wuxi, China.

出版信息

HPB (Oxford). 2021 Jul;23(7):1061-1071. doi: 10.1016/j.hpb.2020.11.002. Epub 2020 Dec 11.

DOI:10.1016/j.hpb.2020.11.002

PMID:33309570

Abstract

BACKGROUND

Tumor microenvironment plays an essential role during the progression of hepatocellular carcinoma (HCC). Tumor infiltrating immune cells (TILs) was an important component of tumor microenvironment. However, whether TIL features are correlated with the prognosis of HCC patients remains unclear.

METHODS

Cancer tissue and paired paracancerous tissues from 220 stage II∼III HBV-related HCC patients were collected. TILs were analyzed using a tyramide signal amplification system combined with immunohistochemistry. Kaplan-Meier survival analysis was conducted to investigate the associations between the prognosis and the infiltrating pattern of TILs.

RESULTS

The patients were classified into three distinct subgroups (Clusters (C)1-3) with different overall survival (OS) and disease-free survival (DFS) according to the distribution pattern of TILs. The CD68/CD8 ratio in the cancer SA was correlated with the prognosis. Patients with a higher CD68/CD8 ratio exhibited poorer OS and DFS than those with a lower ratio. The CD68/CD8 ratio in the cancer SA was an independent factor for OS prediction but not DFS.

CONCLUSION

CD68+ macrophages and CD8+ T-cells are essential immunological determinants for HBV-related HCC prognosis, and the CD68/CD8 ratio in cancer SA is a novel, prognostic factor for OS prediction in HBV-related HCC patients.

摘要

背景

肿瘤微环境在肝细胞癌（HCC）的进展中起着至关重要的作用。肿瘤浸润免疫细胞（TILs）是肿瘤微环境的重要组成部分。然而，TIL 的特征是否与 HCC 患者的预后相关尚不清楚。

方法

收集了 220 例乙型肝炎病毒（HBV）相关的 II∼III 期 HCC 患者的癌组织和配对癌旁组织。使用酪胺信号放大系统结合免疫组织化学法分析 TILs。采用 Kaplan-Meier 生存分析探讨 TIL 浸润模式与预后之间的关系。

结果

根据 TIL 分布模式，患者被分为三个不同的亚组（簇（C）1-3），具有不同的总生存期（OS）和无病生存期（DFS）。癌症 SA 中的 CD68/CD8 比值与预后相关。CD68/CD8 比值较高的患者 OS 和 DFS 较比值较低的患者差。癌症 SA 中的 CD68/CD8 比值是 OS 预测的独立因素，但不是 DFS 的独立因素。