Vitali Čepo Dubravka, Radić Kristina, Turčić Petra, Anić Dora, Komar Barbara, Šalov Mirela
Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia.
Foods. 2020 Dec 9;9(12):1831. doi: 10.3390/foods9121831.
olive pomace extract (OPE) is a rich source of health promoting polyphenols (hydroxytyrosol (HTS) and tyrosol (TS)) and can be used as a nutraceutical ingredient of dietary supplements and functional foods. Its adequate bioavailability is a prerequisite for excreting biological activity and can be significantly and specifically affected by different food matrices.
in order to investigate food effects on polyphenol bioaccessibility, OPE was co-digested with different foods according to internationally harmonized in vitro digestibility method. Impact of particular nutrients on HTS and TS permeability was assessed on Caco-2 cell monolayer.
HTS and TS bioaccessibility and transepithelial permeability can be significantly affected by foods (nutrients), especially by casein and certain types of dietary fiber. Those effects are polyphenol-and nutrient-specific and are achieved either through complexation in gastrointestinal lumen and/or through direct effects of nutrients on intestinal monolayer.
obtained results emphasize the significance and complexity of polyphenol interactions within the food matrix and the necessity of individual investigational approaches with respect to particular food/nutrient and interacting phenolic compounds.
橄榄果渣提取物（OPE）富含具有促进健康作用的多酚类物质（羟基酪醇（HTS）和酪醇（TS）），可作为膳食补充剂和功能性食品的营养成分。其充足的生物利用度是发挥生物活性的前提条件，且会受到不同食物基质的显著且特定的影响。
为研究食物对多酚生物可及性的影响，根据国际统一的体外消化方法，将OPE与不同食物共同消化。在Caco-2细胞单层上评估特定营养素对HTS和TS渗透性的影响。
HTS和TS的生物可及性及跨上皮渗透性会受到食物（营养素）的显著影响，尤其是酪蛋白和某些类型的膳食纤维。这些影响具有多酚和营养素特异性，是通过在胃肠道内形成复合物和/或营养素对肠单层的直接作用实现的。
所得结果强调了食物基质中多酚相互作用的重要性和复杂性，以及针对特定食物/营养素和相互作用的酚类化合物采用个别研究方法的必要性。
