Wagner Alexandra L, Danko Vera, Federle Anna, Klett Daniel, Simon David, Heiss Rafael, Jüngert Jörg, Uder Michael, Schett Georg, Neurath Markus F, Woelfle Joachim, Waldner Maximilian J, Trollmann Regina, Regensburger Adrian P, Knieling Ferdinand
Department of Pediatrics and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, Erlangen, Germany.
Department of Medicine 1, University Hospital Erlangen, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, Erlangen, Germany.
Photoacoustics. 2020 Nov 11;21:100220. doi: 10.1016/j.pacs.2020.100220. eCollection 2021 Mar.
Photo-or optoacoustic imaging (OAI) allows quantitative imaging of target tissues. Using multi-wavelength illumination with subsequent ultrasound detection, it may visualize a variety of different chromophores at centimeter depth. Despite its non-invasive, label-free advantages, the precision of repeated measurements for clinical applications is still elusive. We present a multilayer analysis of n = 1920 imaging datasets obtained from a prospective clinical trial (NCT03979157) in n = 10 healthy adult volunteers. All datasets were analyzed for 13 single wavelengths (SWL) between 660 nm-1210 nm and five MSOT-parameters (deoxygenated/oxygenated/total hemoglobin, collagen and lipid) by a semi-automated batch mode software. Intraclass correlation coefficients (ICC) were good to excellent for intrarater (SWL: 0.82-0.92; MSOT-parameter: 0.72-0.92) and interrater reproducibility (SWL: 0.79-0.87; MSOT-parameter: 0.78-0.86), with the exception for MSOT-parameter lipid (interrater ICC: 0.56). Results were stable over time, but exercise-related effects as well as inter-and intramuscular variability were observed. The findings of this study provide a framework for further clinical OAI implementation.
光声成像（OAI）可对目标组织进行定量成像。通过多波长照明并随后进行超声检测，它可以在厘米深度可视化多种不同的发色团。尽管具有非侵入性、无需标记的优点，但临床应用中重复测量的精度仍难以捉摸。我们对从一项针对n = 10名健康成年志愿者的前瞻性临床试验（NCT03979157）中获得的n = 1920个成像数据集进行了多层分析。所有数据集通过半自动批处理模式软件分析了660 nm至1210 nm之间的13个单波长（SWL）以及五个MSOT参数（脱氧血红蛋白/氧合血红蛋白/总血红蛋白、胶原蛋白和脂质）。组内相关系数（ICC）对于评分者内（SWL：0.82 - 0.92；MSOT参数：0.72 - 0.92）和评分者间的可重复性（SWL：0.79 - 0.87；MSOT参数：0.78 - 0.86）良好至优秀，但MSOT参数脂质除外（评分者间ICC：0.56）。结果随时间稳定，但观察到与运动相关的影响以及肌肉间和肌肉内的变异性。本研究的结果为进一步临床实施OAI提供了框架。
