Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Anal Chem. 2021 Jan 19;93(2):1179-1184. doi: 10.1021/acs.analchem.0c04291. Epub 2020 Dec 15.
Simple, rapid, and accurate detection methods for saccharides are potentially applicable to various fields such as clinical and food chemistry. However, the practical applications of on-site analytical methods are still limited. To this end, herein, we propose a 96-well microtiter plate made of paper as a paper-based chemosensor array device (PCSAD) for the simultaneous classification of 12 saccharides and the quantification of fructose and glucose among 12 saccharides. The mechanism of the saccharide detection relied on an indicator displacement assay (IDA) on the PCSAD using four types of catechol dyes, 3-nitrophenylboronic acid, and the saccharides. The design of the PCSAD and the experimental conditions for the IDA were optimized using a central composite design. The chemosensors exhibited clear color changes upon the addition of saccharides on the paper because of the competitive boronate esterification. The color changes were employed for the subsequent qualitative, semiquantitative, and quantitative analyses using an automated algorithm combined with pattern recognition for digital images. A qualitative linear discrimination analysis offered discrimination of 12 saccharides with a 100% classification rate. The semiquantitative analysis of fructose in the presence of glucose was carried out from the viewpoint of food analysis utilizing a support vector machine, resulting in clear discrimination of the various concentrations of fructose. Most importantly, the quantitative detection of fructose in two types of commercial soft drinks was also successfully carried out without sample pretreatments. Thus, the proposed PCSAD can be a powerful method for on-site food analyses that can meet the increasing demand from consumers for sensors of saccharides.
简单、快速、准确的糖检测方法具有潜在的应用价值，可应用于临床和食品化学等多个领域。然而，现场分析方法的实际应用仍然有限。为此，本文提出了一种基于纸的 96 孔微滴定板作为纸基化学传感器阵列器件（PCSAD），用于同时对 12 种糖进行分类，并对 12 种糖中的果糖和葡萄糖进行定量。糖的检测机制依赖于 PCSAD 上的指示剂置换分析（IDA），使用四种儿茶酚染料、3-硝基苯硼酸和糖。通过中心复合设计优化了 PCSAD 的设计和 IDA 的实验条件。由于竞争的硼酯化，当糖添加到纸上时，化学传感器会显示出明显的颜色变化。颜色变化用于随后的定性、半定量和定量分析，使用自动算法结合数字图像的模式识别。定性线性判别分析提供了 100%分类率的 12 种糖的区分。从食品分析的角度出发，利用支持向量机对半定量分析葡萄糖存在时的果糖，能够清晰地区分果糖的各种浓度。最重要的是，无需样品预处理，还成功地对两种商业软饮料中的果糖进行了定量检测。因此，所提出的 PCSAD 可以成为一种强大的现场食品分析方法，以满足消费者对糖传感器日益增长的需求。
