Sasaki Yui, Lyu Xiaojun, Minami Tsuyoshi
Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Front Chem. 2023 Feb 23;11:1134752. doi: 10.3389/fchem.2023.1134752. eCollection 2023.
Here, we propose a printed 96-well microtiter paper-based chemosensor array device (PCSAD) to simultaneously detect metal ions for river water assessment. Colorimetric chemosensors for metal ions have been designed based on molecular self-assembly using off-the-shelf catechol dyes and a phenylboronic acid (PBA) derivative. The colorimetric self-assembled chemosensors consisting of catechol dyes and a PBA derivative on a 96-well microtiter paper substrate demonstrated various color changes according to the disassembly of the ensembles by the addition of nine types of metal ions. An in-house-made algorithm was used to automate imaging analysis and extract color intensities at seven types of color channels from a captured digital image, allowing for rapid data processing. The obtained information-rich inset data showed fingerprint-like colorimetric responses and was applied to the qualitative and quantitative pattern recognition of metal ions using chemometric techniques. The feasibility of the 96-well microtiter PCSAD for environmental assessment has been revealed by the demonstration of a spike-and-recovery test against metal ions in a river water sample.
在此，我们提出一种基于96孔微量滴定纸的印刷化学传感器阵列装置（PCSAD），用于同时检测金属离子以评估河水。基于分子自组装，利用现成的儿茶酚染料和苯基硼酸（PBA）衍生物设计了用于金属离子的比色化学传感器。在96孔微量滴定纸基质上由儿茶酚染料和PBA衍生物组成的比色自组装化学传感器，根据添加九种金属离子后组装体的分解表现出各种颜色变化。使用自制算法自动进行成像分析，并从捕获的数字图像中提取七种颜色通道的颜色强度，从而实现快速数据处理。获得的信息丰富的内嵌数据显示出类似指纹的比色响应，并应用化学计量技术对金属离子进行定性和定量模式识别。通过对河水样品中金属离子进行加标回收试验，揭示了96孔微量滴定PCSAD用于环境评估的可行性。
