红外脉冲激光激活钛微盘器件实现高效细胞内递送。
Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device.
机构信息
Department of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, India.
Department of Electrical Engineering, University of Cambridge, Cambridge CB3 0FA U.K.
出版信息
ACS Biomater Sci Eng. 2020 Oct 12;6(10):5645-5652. doi: 10.1021/acsbiomaterials.0c00785. Epub 2020 Aug 28.
We report infrared (IR) pulse laser-activated highly efficient parallel intracellular delivery by using an array of titanium microdish (TMD) device. Upon IR laser pulse irradiation, a two-dimensional array of TMD device generated photothermal cavitation bubbles to disrupt the cell membrane surface and create transient membrane pores to deliver biomolecules into cells by a simple diffusion process. We successfully delivered the dyes and different sizes of dextran in different cell types with variations of laser pulses. Our platform has the ability to transfect more than a million cells in a parallel fashion within a minute. The best results were achieved for SiHa cells with a delivery efficiency of 96% and a cell viability of around 98% for propidium iodide dye using 600 pulses, whereas a delivery efficiency of 98% and a cell viability of 100% were obtained for dextran 3000 MW delivery using 700 pulses. For dextran 10,000 MW, the delivery efficiency was 92% and the cell viability was 98%, respectively. The device is compact, easy-to-use, and potentially applicable for cellular therapy and diagnostic purposes.
我们报告了一种使用钛微盘（TMD）阵列装置的红外（IR）脉冲激光激活的高效平行细胞内递药方法。在 IR 激光脉冲照射下，TMD 器件的二维阵列会产生光热空化气泡，破坏细胞膜表面并产生瞬时膜孔，通过简单的扩散过程将生物分子递送到细胞内。我们成功地将染料和不同大小的葡聚糖递送到不同类型的细胞中，激光脉冲的变化也有所不同。我们的平台能够在一分钟内以并行的方式转染超过 100 万个细胞。对于 SiHa 细胞，使用 600 个脉冲时，碘化丙啶染料的转染效率达到 96%，细胞活力约为 98%，而使用 700 个脉冲时，葡聚糖 3000 MW 的转染效率达到 98%，细胞活力达到 100%。对于葡聚糖 10,000 MW，转染效率为 92%，细胞活力为 98%。该设备结构紧凑，易于使用，具有潜在的适用于细胞治疗和诊断的用途。
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