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[利用红外光谱法研究酵母细胞壁的化学成分]

[Use of infrared spectroscopy for studying the chemical composition of yeast cell walls].

作者信息

Guliaeva N D, Zaslevskiĭ B Iu, Rogozkin S V, Liapunova T S

出版信息

Mikrobiologiia. 1977 Jul-Aug;46(4):667-71.

PMID:333237
Abstract

Infrared spectra of intact cells and cell walls of several yeast cultures were recorded by means of immersion of a freeze-dried sample in KBr. The relative intensity and position of absorption bands were analysed, and the bands were related to the respective components of cell walls. The data of IR spectra of various yeast cells were compared to draw conclusions about the chemical composition of their cell walls (the content of protein-peptide and lipid components). The chemical composition of the cell walls changed if one and the same culture was grown on different media.

摘要

通过将冻干样品浸入溴化钾中,记录了几种酵母培养物完整细胞和细胞壁的红外光谱。分析了吸收带的相对强度和位置,并将这些谱带与细胞壁的各个成分相关联。比较了各种酵母细胞的红外光谱数据,以得出关于其细胞壁化学组成(蛋白质 - 肽和脂质成分的含量)的结论。如果将同一培养物在不同培养基上培养,细胞壁的化学组成会发生变化。

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