• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

糖原磷酸化酶B受miR101-3p调控并促进肝细胞癌肿瘤发生。

Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.

作者信息

Cui Guangying, Wang Huifen, Liu Wenli, Xing Jiyuan, Song Wengang, Zeng Zhaohai, Liu Liwen, Wang Haiyu, Wang Xuemei, Luo Hong, Leng Xiaoyang, Shen Shen

机构信息

Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Gene Hospital of Henan Province, Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

出版信息

Front Cell Dev Biol. 2020 Nov 25;8:566494. doi: 10.3389/fcell.2020.566494. eCollection 2020.

DOI:10.3389/fcell.2020.566494
PMID:33324633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7723997/
Abstract

Glycogen metabolism plays a key role in tumorigenesis. High expression levels of glycogen phosphorylase B (PYGB) were reported in several cancers and might be served as a prognostic biomarker for cancer from precancerous lesions. Previous studies indicated the high expression of PYGB in hepatocellular carcinoma (HCC) tissues. However, the detailed roles of PYGB in HCC, as well as the regulatory mechanisms, are still unclear. In this study, we confirmed that PYGB was overexpressed in HCC tissues. PYGB overexpression was significantly associated with an aggressive tumor phenotype and poor prognosis of HCC patients. Functionally, PYGB knockdown suppressed HCC cell proliferation, migration and invasion , as well as tumorigenesis and metastasis . Bioinformatics analysis indicated that PYGB overexpression might enhance epithelial to mesenchymal transition (EMT) in HCC. Moreover, miR-101-3p was identified to post-transcriptionally inhibit the expression of PYGB via binding to 3'-UTR of PYGB. Overexpression of PYGB antagonized the regulatory effect of miR-101-3p on cell proliferation, migration and invasion in HCC cells. In summary, our results suggest that miR-101-3p/PYGB axis has an important role in HCC and PYGB could be served as a novel prognostic biomarker and therapeutic target for improving the prognosis of HCC patients.

摘要

糖原代谢在肿瘤发生过程中起着关键作用。据报道，糖原磷酸化酶B（PYGB）在多种癌症中高表达，可能作为癌前病变向癌症发展的预后生物标志物。先前的研究表明PYGB在肝细胞癌（HCC）组织中高表达。然而，PYGB在HCC中的具体作用以及调控机制仍不清楚。在本研究中，我们证实PYGB在HCC组织中过表达。PYGB过表达与HCC患者侵袭性肿瘤表型和不良预后显著相关。在功能上，敲低PYGB可抑制HCC细胞增殖、迁移和侵袭，以及肿瘤发生和转移。生物信息学分析表明，PYGB过表达可能增强HCC中的上皮-间质转化（EMT）。此外，已确定miR-101-3p通过与PYGB的3'-UTR结合在转录后抑制PYGB的表达。PYGB的过表达拮抗了miR-101-3p对HCC细胞增殖、迁移和侵袭的调控作用。总之，我们的结果表明miR-101-3p/PYGB轴在HCC中起重要作用，并且PYGB可作为改善HCC患者预后的新型预后生物标志物和治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/3ab79a3a968d/fcell-08-566494-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/cc1a531ce78c/fcell-08-566494-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/b5cdb4e5a4d6/fcell-08-566494-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/180cb81c9112/fcell-08-566494-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/a359f84a9fb7/fcell-08-566494-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/47cc286eb41f/fcell-08-566494-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/377ba25fb11e/fcell-08-566494-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/1ae6a1814ffe/fcell-08-566494-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/3ab79a3a968d/fcell-08-566494-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/cc1a531ce78c/fcell-08-566494-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/b5cdb4e5a4d6/fcell-08-566494-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/180cb81c9112/fcell-08-566494-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/a359f84a9fb7/fcell-08-566494-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/47cc286eb41f/fcell-08-566494-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/377ba25fb11e/fcell-08-566494-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/1ae6a1814ffe/fcell-08-566494-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cdf/7723997/3ab79a3a968d/fcell-08-566494-g008.jpg

相似文献

1
Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.糖原磷酸化酶B受miR101-3p调控并促进肝细胞癌肿瘤发生。
Front Cell Dev Biol. 2020 Nov 25;8:566494. doi: 10.3389/fcell.2020.566494. eCollection 2020.
2
Glycogen phosphorylase B promotes ovarian cancer progression via Wnt/β-catenin signaling and is regulated by miR-133a-3p.糖原磷酸化酶 B 通过 Wnt/β-catenin 信号通路促进卵巢癌进展，受 miR-133a-3p 调控。
Biomed Pharmacother. 2019 Dec;120:109449. doi: 10.1016/j.biopha.2019.109449. Epub 2019 Oct 15.
3
HOXA-AS2 Promotes Proliferation and Induces Epithelial-Mesenchymal Transition via the miR-520c-3p/GPC3 Axis in Hepatocellular Carcinoma.HOXA-AS2通过miR-520c-3p/GPC3轴促进肝癌细胞增殖并诱导上皮-间质转化
Cell Physiol Biochem. 2018;50(6):2124-2138. doi: 10.1159/000495056. Epub 2018 Nov 9.
4
Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.肝细胞癌中糖原磷酸化酶同工型的表达、功能及信号传导分析
Oncol Lett. 2022 Jun 7;24(2):244. doi: 10.3892/ol.2022.13364. eCollection 2022 Aug.
5
MiR-23b-3p suppresses epithelial-mesenchymal transition, migration, and invasion of hepatocellular carcinoma cells by targeting c-MET.微小RNA-23b-3p通过靶向c-MET抑制肝癌细胞的上皮-间质转化、迁移和侵袭。
Heliyon. 2022 Oct 17;8(10):e11135. doi: 10.1016/j.heliyon.2022.e11135. eCollection 2022 Oct.
6
Glycogen phosphorylase B promotes cell proliferation and migration through PI3K/AKT pathway in non-small cell lung cancer.糖原磷酸化酶B通过PI3K/AKT途径促进非小细胞肺癌的细胞增殖和迁移。
Exp Lung Res. 2021 Apr;47(3):111-120. doi: 10.1080/01902148.2020.1864065. Epub 2020 Dec 18.
7
Methylation-associated silencing of microRNA-129-3p promotes epithelial-mesenchymal transition, invasion and metastasis of hepatocelluar cancer by targeting Aurora-A.微小RNA-129-3p的甲基化相关沉默通过靶向极光激酶A促进肝细胞癌的上皮-间质转化、侵袭和转移。
Oncotarget. 2016 Nov 22;7(47):78009-78028. doi: 10.18632/oncotarget.12870.
8
Silencing of hsa_circ_0101145 reverses the epithelial-mesenchymal transition in hepatocellular carcinoma via regulation of the miR-548c-3p/LAMC2 axis.hsa_circ_0101145 的沉默通过调节 miR-548c-3p/LAMC2 轴逆转肝癌中的上皮-间充质转化。
Aging (Albany NY). 2020 Jun 18;12(12):11623-11635. doi: 10.18632/aging.103324.
9
MiR-542-3p inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting UBE3C.微小RNA-542-3p通过靶向泛素蛋白连接酶E3C抑制肝细胞癌的转移和上皮-间质转化。
Biomed Pharmacother. 2017 Sep;93:420-428. doi: 10.1016/j.biopha.2017.06.070. Epub 2017 Jun 27.
10
miR-1307-3p promotes tumor growth and metastasis of hepatocellular carcinoma by repressing DAB2 interacting protein.miR-1307-3p 通过抑制 DAB2 相互作用蛋白促进肝癌的肿瘤生长和转移。
Biomed Pharmacother. 2019 Sep;117:109055. doi: 10.1016/j.biopha.2019.109055. Epub 2019 Jun 5.

引用本文的文献

1
Identification of positive cofactor 4 as a diagnostic and prognostic biomarker associated with immune infiltration in hepatocellular carcinoma.鉴定正辅因子4作为与肝细胞癌免疫浸润相关的诊断和预后生物标志物。
ILIVER. 2023 Sep 15;2(4):188-201. doi: 10.1016/j.iliver.2023.08.007. eCollection 2023 Dec.
2
Aberrant expression of PYGB as a potential therapeutic target and its associations with immune cell infiltration in lung cancer.PYGB的异常表达作为一种潜在的治疗靶点及其与肺癌免疫细胞浸润的关联。
Front Immunol. 2025 May 19;16:1536248. doi: 10.3389/fimmu.2025.1536248. eCollection 2025.
3
LncRNA NEAT1 regulation of the miR-101-3p/RAC1 axis affects cervical cancer aerobic glycolysis and progression.

本文引用的文献

1
MicroRNAs Involved in Metastasis of Hepatocellular Carcinoma: Target Candidates, Functionality and Efficacy in Animal Models and Prognostic Relevance.微小 RNA 参与肝细胞癌转移：靶候选物、功能和在动物模型中的疗效以及预后相关性。
Cancer Genomics Proteomics. 2020 Jan-Feb;17(1):1-21. doi: 10.21873/cgp.20163.
2
Glycogen phosphorylase B promotes ovarian cancer progression via Wnt/β-catenin signaling and is regulated by miR-133a-3p.糖原磷酸化酶 B 通过 Wnt/β-catenin 信号通路促进卵巢癌进展，受 miR-133a-3p 调控。
Biomed Pharmacother. 2019 Dec;120:109449. doi: 10.1016/j.biopha.2019.109449. Epub 2019 Oct 15.
3
长链非编码RNA NEAT1对miR-101-3p/RAC1轴的调控影响宫颈癌的有氧糖酵解和进展。
Sci Rep. 2025 May 20;15(1):17436. doi: 10.1038/s41598-025-01698-5.
4
Brain-Type Glycogen Phosphorylase (PYGB) in the Pathologies of Diseases: A Systematic Review.脑型糖原磷酸化酶 (PYGB) 在疾病病理中的作用：系统评价。
Cells. 2024 Feb 5;13(3):289. doi: 10.3390/cells13030289.
5
PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer.PYGL 介导的葡萄糖代谢重编程促进胰腺癌的 EMT 表型和转移。
Int J Biol Sci. 2023 Mar 21;19(6):1894-1909. doi: 10.7150/ijbs.76756. eCollection 2023.
6
Identification of a necroptosis-related gene signature as a novel prognostic biomarker of cholangiocarcinoma.鉴定一个与坏死性凋亡相关的基因特征作为胆管癌的一个新的预后生物标志物。
Front Immunol. 2023 Mar 2;14:1118816. doi: 10.3389/fimmu.2023.1118816. eCollection 2023.
7
Development and clinical validation of a necroptosis-related gene signature for prediction of prognosis and tumor immunity in lung adenocarcinoma.用于预测肺腺癌预后和肿瘤免疫的坏死性凋亡相关基因特征的开发与临床验证
Am J Cancer Res. 2022 Nov 15;12(11):5160-5182. eCollection 2022.
8
MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIF Axis Suppression in the Warburg Effect.微小 RNA101 通过抑制 IDH2/HIF 轴在瓦博格效应中抑制非小细胞肺癌增殖。
Oxid Med Cell Longev. 2022 Oct 18;2022:4938811. doi: 10.1155/2022/4938811. eCollection 2022.
9
miRNA in Molecular Diagnostics.分子诊断中的微小RNA
Bioengineering (Basel). 2022 Sep 9;9(9):459. doi: 10.3390/bioengineering9090459.
10
A Proteomic Platform Unveils the Brain Glycogen Phosphorylase as a Potential Therapeutic Target for Glioblastoma Multiforme.蛋白质组学平台揭示脑糖原磷酸化酶可能成为胶质母细胞瘤的治疗靶点。
Int J Mol Sci. 2022 Jul 25;23(15):8200. doi: 10.3390/ijms23158200.
Breast cancers utilize hypoxic glycogen stores via PYGB, the brain isoform of glycogen phosphorylase, to promote metastatic phenotypes.
乳腺癌通过 PYGB（糖元磷酸化酶的脑同工酶）利用缺氧的糖元储存来促进转移表型。
PLoS One. 2019 Sep 19;14(9):e0220973. doi: 10.1371/journal.pone.0220973. eCollection 2019.
4
Enhancer of zeste 2 polycomb repressive complex 2 subunit promotes sorafenib resistance of hepatocellular carcinoma though insulin-like growth factor 1 receptor.增强子结合锌指蛋白 2 多梳抑制复合物 2 亚基通过胰岛素样生长因子 1 受体促进肝细胞癌对索拉非尼的耐药性。
Anticancer Drugs. 2019 Aug;30(7):e0746. doi: 10.1097/CAD.0000000000000746.
5
Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway.恩度通过 HGF-Met 通路调控肺癌细胞 EMT、迁移和侵袭。
Mol Cell Probes. 2019 Jun;45:57-64. doi: 10.1016/j.mcp.2019.05.003. Epub 2019 May 13.
6
MicroRNA-101-3p suppresses proliferation and migration in hepatocellular carcinoma by targeting the HGF/c-Met pathway.miR-101-3p 通过靶向 HGF/c-Met 通路抑制肝癌细胞的增殖和迁移。
Invest New Drugs. 2020 Feb;38(1):60-69. doi: 10.1007/s10637-019-00766-8. Epub 2019 Mar 30.
7
Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer.长链非编码 RNA PVT1 通过调控 miR-143/HK2 轴促进胆囊癌的进展。
Mol Cancer. 2019 Mar 2;18(1):33. doi: 10.1186/s12943-019-0947-9.
8
Blocking Triggering Receptor Expressed on Myeloid Cells-1-Positive Tumor-Associated Macrophages Induced by Hypoxia Reverses Immunosuppression and Anti-Programmed Cell Death Ligand 1 Resistance in Liver Cancer.阻断低氧诱导的表达在髓系细胞上的触发受体 1 阳性肿瘤相关巨噬细胞可逆转肝癌中的免疫抑制和抗程序性细胞死亡配体 1 耐药性。
Hepatology. 2019 Jul;70(1):198-214. doi: 10.1002/hep.30593. Epub 2019 Apr 12.
9
Lnc-PDZD7 contributes to stemness properties and chemosensitivity in hepatocellular carcinoma through EZH2-mediated ATOH8 transcriptional repression.长链非编码 RNA-PDZD7 通过 EZH2 介导的 ATOH8 转录抑制作用促进肝癌的干性特征和化疗敏感性。
J Exp Clin Cancer Res. 2019 Feb 20;38(1):92. doi: 10.1186/s13046-019-1106-2.
10
Cancer statistics, 2019.癌症统计数据，2019 年。
CA Cancer J Clin. 2019 Jan;69(1):7-34. doi: 10.3322/caac.21551. Epub 2019 Jan 8.