School of Bioscience and Biotechnology, Tokyo University of Technology, Tokyo, Japan E-mail:
School of Health Sciences, Tokyo University of Technology, Tokyo, Japan.
J Water Health. 2020 Dec;18(6):899-910. doi: 10.2166/wh.2020.014.
The increasing prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and carbapenem-resistant Enterobacteriaceae (CRE) is a worldwide health threat. Monitoring of these resistant bacteria in the environment can provide regional prevalence reflecting both healthy and infected populations, although the quantitative monitoring of those resistant bacteria, especially CRE, is difficult due to their low proportion in the total Enterobacteriaceae population and the possible interference by autochthonous species with intrinsic resistance. In this study, these resistant bacteria in treated wastewater were quantified at 12 different treatment plants. The proportions of cefotaxime-resistant and ESBL-producing E. coli in the total E. coli population in the chlorinated effluents in Tokyo were 5.7 and 5.3%, respectively. The estimated proportion of CRE was 0.007% with the constituting species of Klebsiella spp. and Enterobacter spp., although the conditions during the first incubation may have affected the estimation even after the correction by the proportion of resistant population in the isolates. The observed resistant proportions in this study were lower than those in the surveillance on nosocomial infection not only for inpatients but also for outpatients, and higher than those in the veterinary monitoring.
产超广谱β-内酰胺酶（ESBL）的大肠杆菌和耐碳青霉烯类肠杆菌科（CRE）的日益流行是全球健康威胁。监测环境中的这些耐药菌可以提供反映健康和感染人群的区域流行情况，尽管由于其在总肠杆菌科群体中的比例较低，以及可能存在具有内在耐药性的土著物种的干扰，对这些耐药菌，尤其是 CRE 的定量监测具有一定难度。在本研究中，在 12 个不同的处理厂对处理废水中的这些耐药菌进行了定量检测。在东京加氯处理废水中，头孢他啶耐药和产 ESBL 的大肠杆菌在总大肠杆菌群体中的比例分别为 5.7%和 5.3%。估计 CRE 的比例为 0.007%，其构成种为肺炎克雷伯菌和肠杆菌属，尽管在第一次孵育时的条件可能会影响即使在通过分离物中耐药群体的比例进行校正后仍会产生的估计值。本研究中观察到的耐药比例低于医院感染监测中不仅包括住院患者而且包括门诊患者的比例，也高于兽医监测中的比例。
