Martins Pedro M, Navarro Susanna, Silva Alexandra, Pinto Maria F, Sárkány Zsuzsa, Figueiredo Francisco, Pereira Pedro José Barbosa, Pinheiro Francisca, Bednarikova Zuzana, Burdukiewicz Michał, Galzitskaya Oxana V, Gazova Zuzana, Gomes Cláudio M, Pastore Annalisa, Serpell Louise C, Skrabana Rostislav, Smirnovas Vytautas, Ziaunys Mantas, Otzen Daniel E, Ventura Salvador, Macedo-Ribeiro Sandra
Instituto de Biologia Molecular e Celular and Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.
Front Mol Neurosci. 2020 Nov 27;13:582488. doi: 10.3389/fnmol.2020.582488. eCollection 2020.
Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.
关于多种蛋白质和易聚集肽的相分离和淀粉样蛋白形成的报告经常被用于探索与几种人类疾病相关的分子机制。这些报告所传达的信息可直接用于转化研究，例如用于设计更好的药物筛选策略，或汇编到数据库中以对新型聚集预测算法进行基准测试。鉴于微小的实验方案差异会决定蛋白质聚集测定的不同结果，因此强烈需要对不同类型的聚集体以及用于其产生的详细方法进行标准化描述。为了满足这一需求，我们综合了蛋白质自组装和聚集的第一性原理及已发表文献，制定了可重复聚集实验所需的最低信息（MIRRAGGE）指南。这一共识性信息旨在涵盖实验可重复性的主要和细微决定因素，同时避免过多对非专业用户实际意义有限的技术细节。MIRRAGGE表格（补充信息中有模板）可作为设计新研究的指南以及提交实验报告以供发表时的核对清单。充分披露相关信息还能使其他研究人员正确重现结果，并便于将系统数据存入经过整理的数据库。
