College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
Chem Asian J. 2021 Feb 15;16(4):277-281. doi: 10.1002/asia.202001325. Epub 2021 Jan 12.
Hypochlorous acid (HOCl) plays a crucial role in many physiological processes and is widely used as bleach, deodorant and fungicide. In this work, we designed an amphiphilic hydrazone fluorescent molecule THG-1 containing hydrophilic sugar units and hydrophobic tetraphenylethylene unit for ratiometric detection of HOCl with high sensitivity and excellent selectivity based on HOCl-triggered hydrolyzation reaction and aggregation-induced emission (AIE) effect. The detection mechanism was verified by liquid chromatograph mass spectrometry experiments and scanning electron microscope (SEM) tests. Contrast experiments revealed that the numbers of lactose unit and hydrazone linker were essential for assembly of THG-1 and detection of HOCl. In addition, THG-1 was successfully used for imaging of exogenous and endogenous HOCl in living cells.
次氯酸（HOCl）在许多生理过程中发挥着关键作用，被广泛用作漂白剂、除臭剂和杀菌剂。在这项工作中，我们设计了一种两亲性腙荧光分子 THG-1，它含有亲水性糖单元和疏水性四苯乙烯单元，基于 HOCl 触发的水解反应和聚集诱导发射（AIE）效应，用于高灵敏度和优异选择性的 HOCl 比率检测。通过液相色谱-质谱实验和扫描电子显微镜（SEM）测试验证了检测机制。对比实验表明，乳糖单元和腙连接体的数量对于 THG-1 的组装和 HOCl 的检测至关重要。此外，THG-1 成功地用于活细胞中外源和内源性 HOCl 的成像。
