School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, Guangdong 510006, China.
Department of Chemistry and Center of Super-Diamond and Advanced Films (COSDAF), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China.
J Mater Chem B. 2019 Sep 7;7(33):5125-5131. doi: 10.1039/c9tb01279f. Epub 2019 Jul 31.
An ultra-sensitive and ratiometric fluorescent probe for hypochlorous acid (HOCl) detection based on the mechanism of aggregation induced emission (AIE) and through-bond energy transfer (TBET) has been reported herein. By exploiting the advantages of AIE and TBET, which eliminates emission leakage from dark donors, the probe exhibits ultra-high sensitivity towards HOCl by an enhancement of over 7000-fold in the fluorescence ratio (I/I), which is one of the highest recorded so far. The reaction mechanism has been discussed in detail, and the effects of interferents and the reaction kinetics have also been investigated. Lastly, the successful result of exogenous/endogenous HOCl imaging detection in different cell lines indicates the potential use of the probe in living systems.
本文报道了一种基于聚集诱导发射（AIE）和键间能量转移（TBET）机制的超灵敏、比率型荧光探针用于检测次氯酸（HOCl）。利用 AIE 和 TBET 的优势，可以消除暗施主的发射泄漏，探针对 HOCl 表现出超高的灵敏度，荧光比（I/I）增强超过 7000 倍，这是迄今为止记录到的最高值之一。详细讨论了反应机理，并研究了干扰物的影响和反应动力学。最后，在不同细胞系中外源/内源性 HOCl 成像检测的成功结果表明了该探针在活体系统中的潜在应用。
