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大鼠肾脏衰老过程中氧化应激的体视学研究与分析

Stereological study and analysis of oxidative stress during renal aging in rats.

作者信息

Melchioretto Eduardo Felippe, Zeni Marcelo, Veronez Djanira Aparecida da Luz, Filipak Neto Francisco, Digner Ingridy de Souza, Fraga Rogerio de

机构信息

Fellow PhD degree, Postgraduate Program in Surgical Clinic, Universidade Federal do Paraná, Curitiba - PR, Brazil. Conception and design, manuscript preparation and writing, critical revision.

Fellow Master degree, Department of Urology, Medical School, Universidade Federal da Fronteira Sul, Chapecó - SC, Brazil. Design.

出版信息

Acta Cir Bras. 2020 Dec 18;35(11):e351106. doi: 10.1590/ACTA351106. eCollection 2020.

DOI:10.1590/ACTA351106
PMID:33331456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7748077/
Abstract

PURPOSE

To evaluate renal histological changes by stereology and morphometry and analyze the main markers of oxidative stress in rats undergoing natural aging.

METHODS

Seventy two Wistar rats were divided into six groups of 12 rats each, which were euthanized at 3, 6, 9, 12, 18, and 24 months of age. Right kidney was stereologically and morphometrically analyzed to calculate the volumetric density (Vv[glom]), numerical density (Nv[glom]) and glomerular volume (Vol[glom]). Left kidney was used to determine the levels of nonprotein thiols, lipid peroxidation, and protein carbonylation, as well as the activities of superoxide-dismutase and catalase enzymes.

RESULTS

Both Vv[glom] and Nv[glom] values showed gradual decreases between groups. Activity of superoxide-dismutase was elevated at 24 months of age, and the levels of nonprotein thiols were higher in older animals. Greater catalase activity and protein carbonylation were observed in animals between 6 and 12 months of age but lessened in older rats. Lipid peroxidation decreased in the older groups.

CONCLUSIONS

Morphometric and stereological analyses revealed a gradual decrease in the volume and density of renal glomeruli during aging, as well as kidney atrophy. These findings related to oxidative stress clarify many changes occurring in kidney tissues during senescence in rats.

摘要

目的

通过体视学和形态计量学评估自然衰老大鼠的肾脏组织学变化，并分析氧化应激的主要标志物。

方法

将72只Wistar大鼠分为6组，每组12只，分别在3、6、9、12、18和24月龄时实施安乐死。对右侧肾脏进行体视学和形态计量学分析，以计算体积密度（Vv[glom]）、数量密度（Nv[glom]）和肾小球体积（Vol[glom]）。左侧肾脏用于测定非蛋白硫醇、脂质过氧化和蛋白质羰基化水平，以及超氧化物歧化酶和过氧化氢酶的活性。

结果

Vv[glom]和Nv[glom]值在各年龄组之间均呈逐渐下降趋势。超氧化物歧化酶活性在24月龄时升高，非蛋白硫醇水平在老年动物中更高。在6至12月龄的动物中观察到较高的过氧化氢酶活性和蛋白质羰基化，但在老年大鼠中有所降低。脂质过氧化在老年组中减少。

结论

形态计量学和体视学分析显示，衰老过程中肾小球的体积和密度逐渐减小，同时伴有肾脏萎缩。这些与氧化应激相关的发现阐明了大鼠衰老过程中肾脏组织发生的许多变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/5aa5d55db19d/1678-2674-acb-35-11-e351106-gf09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/2b00900acd5d/1678-2674-acb-35-11-e351106-gf01.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/758b5fcacdf3/1678-2674-acb-35-11-e351106-gf03.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/5aa5d55db19d/1678-2674-acb-35-11-e351106-gf09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/2b00900acd5d/1678-2674-acb-35-11-e351106-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/201f17950978/1678-2674-acb-35-11-e351106-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/758b5fcacdf3/1678-2674-acb-35-11-e351106-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/65781962a19c/1678-2674-acb-35-11-e351106-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/e60961cdbf68/1678-2674-acb-35-11-e351106-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/025d7d9a9ddc/1678-2674-acb-35-11-e351106-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/9c4fa37310b3/1678-2674-acb-35-11-e351106-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/60146438fe81/1678-2674-acb-35-11-e351106-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a9b6/7748077/5aa5d55db19d/1678-2674-acb-35-11-e351106-gf09.jpg

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