Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.
Biomedicine undergraduate course, Universidade Nove de Julho (UNINOVE), Sao Paulo, Brazil.
Photobiomodul Photomed Laser Surg. 2021 Jan;39(1):30-37. doi: 10.1089/photob.2019.4782. Epub 2020 Dec 17.
To evaluate the effect of photobiomodulation (PBM) on cell viability, synthesis of nitric oxide (NO), and interleukin (IL)-6 inflammatory cytokine production in myoblasts cultured in the presence of lipopolysaccharides (LPSs). C2C12 myoblasts were treated with LPS and PBM using different parameters (wavelength: 780 nm; beam spot: 0.04 cm; power output: 10 or 40 mW; energy density: 5 or 20 J/cm; and 20-sec exposure time). Nonirradiated cells were used to the control group. An increase in cell viability was found in both LPS groups in comparison with the control. PBM with the higher power output (40 mW) induced a reduction in cell viability. PBM also modulated the synthesis of NO in the myoblasts, but did not alter the expression of IL-6. Based on these findings, PBM is capable of modulating the cell viability and the production of NO in LPS-treated myoblasts and it is, therefore, a possible tool for the treatment of muscle injury caused by infection.
为了评估在脂多糖（LPS）存在的情况下，光生物调节（PBM）对肌母细胞活力、一氧化氮（NO）合成和白细胞介素（IL）-6 炎性细胞因子产生的影响。将 C2C12 肌母细胞用不同参数的 LPS 和 PBM 处理（波长：780nm；光斑：0.04cm；功率输出：10 或 40mW；能量密度：5 或 20J/cm；暴露时间：20 秒）。未辐照的细胞用作对照组。与对照组相比，两种 LPS 组的细胞活力均增加。与较低功率输出（40mW）相比，较高功率输出（40mW）的 PBM 诱导细胞活力降低。PBM 还调节了肌母细胞中 NO 的合成，但未改变 IL-6 的表达。基于这些发现，PBM 能够调节 LPS 处理的肌母细胞中的细胞活力和 NO 的产生，因此，它可能是治疗感染引起的肌肉损伤的一种手段。
