Mesquita-Ferrari Raquel Agnelli, Alves Agnelo Neves, de Oliveira Cardoso Vinicius, Artilheiro Paola Pelegrineli, Bussadori Sandra Kalil, Rocha Lilia Alves, Nunes Fábio Daumas, Fernandes Kristianne Porta Santos
Postgraduate Program in Rehabilitation Sciences and Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235/249, Liberdade, São Paulo, SP, 01504-001, Brazil.
Departament of Molecular Pathology, School of Dentistry, University of São Paulo, Av. Professor Lineu Prestes, 2227, Cidade Universitária, São Paulo, 05508-000, SP, Brazil.
Lasers Med Sci. 2015 Nov;30(8):2209-13. doi: 10.1007/s10103-015-1715-8. Epub 2015 Jan 24.
Low-level laser irradiation (LLLI) is increasingly used to treat musculoskeletal disorders, with satisfactory results described in the literature. Skeletal muscle satellite cells play a key role in muscle regeneration. The aim of the present study was to evaluate the effect of LLLI on cell viability, creatine kinase (CK) activity, and the expression of myogenic regulatory factors in C2C12 myoblasts during the differentiation process. C2C12 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 2% horse serum and submitted to irradiation with GaAlAs diode laser (wavelength, 780 nm; output power, 10 mW; energy density, 5 J/cm2). Cell viability and the expression of myogenic regulatory factors were assessed 24, 48, and 72 h after irradiation by 3-(4,5-dimethylthiazol-2-yl)-2,5,-diphenyltetrazolium bromide (MTT) assay and quantitative real-time polymerase chain reaction (RT-qPCR), respectively. CK activity was analyzed at 24 and 72 h. An increase in cell viability was found in the laser group in comparison to the control group at all evaluation times. CK activity was significantly increased in the laser group at 72 h. Myogenin messenger RNA (mRNA) demonstrated a tendency toward an increase in the laser group, but the difference in comparison to the control group was non-significant. In conclusion, LLLI was able to modulate cell viability and CK activity in C2C12 myoblasts during the differentiation process.
低强度激光照射（LLLI）越来越多地用于治疗肌肉骨骼疾病，文献中描述了令人满意的结果。骨骼肌卫星细胞在肌肉再生中起关键作用。本研究的目的是评估LLLI对C2C12成肌细胞在分化过程中细胞活力、肌酸激酶（CK）活性和生肌调节因子表达的影响。C2C12细胞在含有2%马血清的杜氏改良 Eagle 培养基（DMEM）中培养，并用GaAlAs二极管激光（波长780 nm；输出功率10 mW；能量密度5 J/cm²）进行照射。分别在照射后24、48和72小时，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和定量实时聚合酶链反应（RT-qPCR）评估细胞活力和生肌调节因子的表达。在24和72小时分析CK活性。在所有评估时间，与对照组相比，激光组细胞活力增加。激光组在72小时时CK活性显著增加。肌生成素信使核糖核酸（mRNA）在激光组有增加的趋势，但与对照组相比差异不显著。总之，LLLI能够在分化过程中调节C2C12成肌细胞的细胞活力和CK活性。
