Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
National Biodiversity Institute of Korea, Seocheon, Chungcheongnam-do 33662, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004603. Epub 2020 Dec 11.
A Gram-stain-negative, orange-pigmented and strictly aerobic bacterium, designated strain MJ115, was isolated from seawater in Pohang, South Korea. Cells were non-motile rods and showed positive reactions for catalase and oxidase tests. Growth of strain MJ115 was observed at 4-35 °C (optimum, 30 °C), pH 6.0-7.0 (optimum, pH 6.5) and in the presence of 0-8.0 % (w/v) NaCl (optimum, 2.0%). Strain MJ115 contained iso-C, anteiso-C, anteiso-C 9 C 2-OH, iso-C 3-OH, iso-C 3-OH and summed feature 3 (C 7 and/or C 6) as major cellular fatty acids and menaquinone-6 as the major respiratory quinone. Phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids were detected as major polar lipids. The G+C content of the genomic DNA was 40.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ115 formed a phyletic lineage with S1-08, JC2678 and AKS 622 within the genus . Strain MJ115 was also most closely related to S1-08, JC2678 and AKS 622 with 96.5, 96.4 and 96.0 % 16S rRNA sequence similarities, respectively. Here it is proposed that strain MJ115 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain is MJ115 (=KCTC 72237=NBRC 113963). In addition, the comparison of the whole genome sequences and phenotypic features suggested that and belong to the same species. Therefore, it is proposed that is reclassified as a later heterotypic synonym of .
一株革兰氏阴性、橙色着色、严格需氧的细菌,被命名为 MJ115 菌株,从韩国浦项的海水中分离得到。细胞为非运动杆状,过氧化氢酶和氧化酶试验呈阳性反应。MJ115 菌株的生长在 4-35°C(最佳温度为 30°C)、pH 值 6.0-7.0(最佳 pH 值为 6.5)和 0-8.0%(w/v)NaCl(最佳 2.0%)的条件下观察到。菌株 MJ115 含有 iso-C、anteiso-C、anteiso-C 9 C 2-OH、iso-C 3-OH、iso-C 3-OH 和总和特征 3(C 7 和/或 C 6)作为主要细胞脂肪酸和menaquinone-6 作为主要呼吸醌。鉴定出磷脂酰乙醇胺、两种未鉴定的胺脂和四种未鉴定的脂类作为主要极性脂类。基因组 DNA 的 G+C 含量为 40.7 mol%。基于 16S rRNA 基因序列的系统发育分析表明,菌株 MJ115 与属内的 S1-08、JC2678 和 AKS 622 一起形成一个进化枝。菌株 MJ115 与 S1-08、JC2678 和 AKS 622 的 16S rRNA 序列相似性最高,分别为 96.5%、96.4%和 96.0%。因此,建议菌株 MJ115 代表一个新的属种,命名为 sp. nov.。其模式菌株为 MJ115(=KCTC 72237=NBRC 113963)。此外,全基因组序列和表型特征的比较表明, 和 属于同一物种。因此,建议将 重新分类为 的同物异名。