Division of Biochemistry, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Genomics and Bioinformatics Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
PLoS One. 2020 Dec 17;15(12):e0244030. doi: 10.1371/journal.pone.0244030. eCollection 2020.
Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1::GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1-3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S::GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant (aba2-1) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.
非生物胁迫，特别是盐度、干旱和高温，导致作物产量大幅下降。在这项研究中，我们鉴定了 AT4G18280 注释为富含甘氨酸的细胞壁蛋白样（以下简称 GRPL1）蛋白，作为一个潜在的多胁迫响应基因。对公共转录组数据的分析和 pGRPL1::GUS 的 GUS 分析表明，GRPL1 在干旱、盐度和热胁迫下强烈诱导。过表达 GRPL1-3HA 的转基因植物在盐胁迫下表现出明显更高的萌发率、根伸长率和存活率。此外，35S::GRPL1-3HA 转基因系在干旱和热胁迫下也表现出更高的存活率。GRPL1 在不同生长和胁迫条件下与脱落酸（ABA）途径基因表现出相似的表达模式，这表明 GRPL1 可能作用于 ABA 途径，这进一步得到 ABA 缺陷突变体（aba2-1）在干旱胁迫下无法诱导 GRPL1 的事实的支持。总之，我们的数据表明 GRPL1 是一个潜在的多胁迫响应基因，作用于 ABA 途径的下游。
