Wang Meng, Zhu Qiang, Li Xiaoxu, Hu Jinhong, Song Fan, Liang Wangli, Ma Xiaorong, Wang Lingxia, Liang Wenyu
School of Life Sciences, Ningxia University, Yinchuan 750021, China.
Foods. 2022 Jun 18;11(12):1798. doi: 10.3390/foods11121798.
is a kind of terrestrial edible cyanobacteria with important ecological and economic value which has developed special mechanisms to adapt to drought conditions. However, the specific mechanism of lipidome changes in drought tolerance of has not been well understood. In this study, the ultra-high-performance liquid chromatography and mass spectrometry were employed to analyze the lipidome changes of under dehydration. A total of 853 lipid molecules were identified, of which 171 were significantly different from that of the control group. The digalactosyldiacylglycerol/monogalactosyldiacylglycerol (DGDG/MGDG) ratio was increased. The amount of wax ester (WE) was sharply decreased during drought stress, while Co (Q10) was accumulated. The levels of odd chain fatty acids (OCFAs) were increased under dehydration, positively responding to drought stress according to the energy metabolism state. In conclusion, the lipidomic data corroborated that oxidation, degradation, and biosynthesis of membrane lipids took place during lipid metabolism, which can respond to drought stress through the transformation of energy and substances. Besides, we constructed a lipid metabolic model demonstrating the regulatory mechanism of drought stress in . The present study provides insight into the defense strategies of cyanobacteria in lipid metabolic pathways.
是一种具有重要生态和经济价值的陆生可食用蓝细菌，其已发展出特殊机制以适应干旱条件。然而，其耐旱性中脂质组变化的具体机制尚未得到充分了解。在本研究中，采用超高效液相色谱和质谱分析脱水条件下的脂质组变化。共鉴定出853种脂质分子，其中171种与对照组有显著差异。二半乳糖基二酰基甘油/单半乳糖基二酰基甘油（DGDG/MGDG）比值增加。干旱胁迫期间蜡酯（WE）含量急剧下降，而辅酶Q10（Co(Q10)）积累。脱水条件下奇数链脂肪酸（OCFAs）水平升高，根据能量代谢状态对干旱胁迫呈正响应。总之，脂质组学数据证实脂质代谢过程中发生了膜脂的氧化、降解和生物合成，其可通过能量和物质的转化来应对干旱胁迫。此外，我们构建了一个脂质代谢模型，展示了干旱胁迫在中的调控机制。本研究为蓝细菌在脂质代谢途径中的防御策略提供了见解。
