Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, China.
J Fish Dis. 2021 Mar;44(3):263-271. doi: 10.1111/jfd.13320. Epub 2020 Dec 17.
In this study, 1,800 turbot (Scophthalmus maximus) individuals from 30 full-sib families were experimentally infected with Vibrio anguillarum, and the expression levels of the immune factors lysozyme, hepcidin, heat-shock protein 70 (HSP70), HSP90, immunoglobulin M (IgM), C-type lectin and Lily-type lectin in the liver were measured by real-time PCR. Heritability values of the seven immune factors were 0.289 ± 0.087, 0.092 ± 0.024, 0.282 ± 0.043, 0.244 ± 0.027, 0.343 ± 0.081, 0.092 ± 0.011 and 0.084 ± 0.009, respectively. The ranges of phenotypic, genetic and environmental correlations were -0.889 to 0.759, -0.841 to 0.888 and -0.919 to 0.883, respectively. The heritability values of HSP70, HSP90 and IgM were moderate, and the genetic correlations between HSP70, HSP90 and IgM were moderate to highly positive, which suggests that the immunocompetence of turbot against V. anguillarum can be improved by genetically improving these three immune characters via multi-trait integrated breeding technology or indirect selection.
在这项研究中，我们用鳗弧菌对 30 个全同胞家系的 1800 尾大菱鲆进行了人工感染实验，并通过实时 PCR 测定了肝组织中溶菌酶、铁调素、热休克蛋白 70（HSP70）、热休克蛋白 90（HSP90）、免疫球蛋白 M（IgM）、C 型凝集素和百合型凝集素这 7 种免疫因子的表达水平。这 7 种免疫因子的遗传力估计值分别为 0.289±0.087、0.092±0.024、0.282±0.043、0.244±0.027、0.343±0.081、0.092±0.011 和 0.084±0.009。表型、遗传和环境相关的范围分别为-0.889 到 0.759、-0.841 到 0.888 和-0.919 到 0.883。HSP70、HSP90 和 IgM 的遗传力估计值处于中等水平，且 HSP70、HSP90 和 IgM 之间的遗传相关性从中等到高度正相关，这表明通过多性状综合选育技术或间接选择，遗传改良这些 3 种免疫特性可提高大菱鲆对鳗弧菌的免疫能力。
