Kelly S L, Parry J M
School of Biological Sciences, University College of Swansea, UK.
Mutagenesis. 1986 Mar;1(2):151-5. doi: 10.1093/mutage/1.2.151.
Enhanced mutagenic action after methylmethanesulphonate (MMS) treatment was found in pre-replicative meiotic yeast cells of the strain D7. The level of gene conversion after MMS treatment rose above the spontaneous level during the period of commitment to meiotic recombination, but at later times into meiosis became indistinguishable from the full meiotic level. In contrast reciprocal recombination detected between ade 2 and the centromere of chromosome XV after MMS treatment remained above the spontaneous level up to commitment to meiotic cell division with relatively high levels through meiotic prophase I. These results are discussed in relation to MMS-induced damage and its repair, particularly double-strand break repair.
在D7菌株的减数分裂前复制酵母细胞中，发现甲磺酸甲酯（MMS）处理后诱变作用增强。在减数分裂重组的起始阶段，MMS处理后的基因转换水平高于自发水平，但在减数分裂后期则与完全减数分裂水平无法区分。相比之下，MMS处理后在ade 2和第十五号染色体着丝粒之间检测到的 reciprocal 重组在减数分裂细胞分裂起始前一直高于自发水平，在减数分裂前期I阶段保持相对较高水平。结合MMS诱导的损伤及其修复，特别是双链断裂修复对这些结果进行了讨论。
