Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 15;249:119241. doi: 10.1016/j.saa.2020.119241. Epub 2020 Nov 28.
The present work describes development of rapid, robust, sensitive and green spectrofluorimetric method for determination of favipiravir (FAV). Different factors affecting fluorescence were carefully studied and Box Behnken Design was applied to optimize experimental parameters. The proposed method is based on measuring native fluorescence of FAV in 0.2 M borate buffer (pH 8.0) at 432 nm after excitation at 361 nm. There was a linear relationship between FAV concentration and relative fluorescence intensity over the range 40-280 ng/mL with limit of detection of 9.44 ng/mL and quantitation limit of 28.60 ng/mL. The method was successfully implemented for determination of FAV in its pharmaceutical formulation with mean % recovery of 99.26 ± 0.87. Moreover, the high sensitivity of the method allowed determination of FAV in spiked human plasma over a range of 48-192 ng/mL. The proposed spectrofluorimetric method was proved to be eco-friendly according to analytical eco-scale.
本工作描述了一种快速、灵敏、绿色的荧光分光光度法测定法维拉韦（FAV）的方法。仔细研究了影响荧光的不同因素，并应用 Box-Behnken 设计对实验参数进行了优化。该方法基于在 0.2 M 硼酸盐缓冲液（pH 8.0）中测量 FAV 的本征荧光，在 361nm 激发下于 432nm 处测定。FAV 浓度与相对荧光强度在 40-280ng/mL 范围内呈线性关系，检出限为 9.44ng/mL，定量限为 28.60ng/mL。该方法成功地用于测定其药物制剂中的 FAV，平均回收率为 99.26±0.87%。此外，该方法的高灵敏度允许在 48-192ng/mL 的范围内测定人血浆中加标的 FAV。根据分析生态标度，所提出的荧光分光光度法被证明是环保的。
