School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430205, China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Int J Mol Sci. 2020 Dec 15;21(24):9529. doi: 10.3390/ijms21249529.
Pollen grain was explored as a new carrier for enzyme immobilization. After being modified with boric acid-functionalized titania, the pollen grain was able to covalently immobilize glycosylated enzymes by boronate affinity interaction under very mild experimental conditions (e.g., pH 7.0, ambient temperature and free of organic solvent). The glucose oxidase and horse radish peroxidase-immobilized pollen grain became a bienzyme system. The pollen grain also worked as an indicator of the cascade reaction by changing its color. A rapid, simple and cost-effective approach for the visual detection of glucose was then developed. When the glucose concentration exceeded 0.5 mM, the color change was observable by the naked eye. The assay of glucose in body fluid samples exhibited its great potential for practical application.
花粉粒被探索作为酶固定化的一种新载体。经过硼酸功能化二氧化钛修饰后，花粉粒可以在非常温和的实验条件下（例如，pH 值为 7.0、环境温度和无有机溶剂）通过硼酸亲和相互作用共价固定糖基化酶。葡萄糖氧化酶和辣根过氧化物酶固定化的花粉粒成为双酶系统。花粉粒还通过改变颜色作为级联反应的指示剂。然后开发了一种快速、简单和具有成本效益的方法用于葡萄糖的可视化检测。当葡萄糖浓度超过 0.5mM 时，肉眼即可观察到颜色变化。体液样品中葡萄糖的测定显示了其在实际应用中的巨大潜力。
