葡萄糖氧化酶和过氧化物酶的固定化及其在血清葡萄糖流动注射分析中的应用。

Immobilization of glucose oxidase and peroxidase and their application in flow-injection analysis for glucose in serum.

作者信息

Guo J A, Mo P S, Li G X

机构信息

National Center for Clinical Laboratory, Beijing, People's Republic of China.

出版信息

Appl Biochem Biotechnol. 1990 Jan;23(1):15-24. doi: 10.1007/BF02942049.

DOI:10.1007/BF02942049

PMID:2105696

Abstract

Glucose oxidase (GOD) and Horseradish peroxidase (HRP) were covalently coupled to alkylamine controlled pore glass by means of glutaraldehyde. About 700-800 U/g of immobilized GOD and 300-400 U/g of immobilized HRP were obtained. Some factors of affecting enzyme immobilization were discussed. The immobilized enzymes were packed into a plastic tube and used in flow-injection analysis (FIA) for glucose in serum. A good linearity range was observed for this immobilized enzyme system at 20 mg/mL to 1000 mg/dL D-glucose, the recovery was 95.4-103.5%, the within-batch imprecision was 0.8-2.2%, and the between-batch imprecision was 2.2-4.2%. More than 100 samples were measured within an hour. One enzyme column with five units of immobilized GOD and HRP, applied for 50 assays/d, has been used for more than 2 mo.

摘要

葡萄糖氧化酶（GOD）和辣根过氧化物酶（HRP）通过戊二醛共价偶联到烷基胺控制孔径玻璃上。获得了约700 - 800 U/g的固定化GOD和300 - 400 U/g的固定化HRP。讨论了影响酶固定化的一些因素。将固定化酶装入塑料管中，用于血清中葡萄糖的流动注射分析（FIA）。该固定化酶系统在20 mg/mL至1000 mg/dL D - 葡萄糖范围内观察到良好的线性范围，回收率为95.4 - 103.5%，批内不精密度为0.8 - 2.2%，批间不精密度为2.2 - 4.2%。一小时内可测量100多个样品。一个含有五个单位固定化GOD和HRP的酶柱，每天用于50次测定，已使用超过2个月。