Liaquat Fiza, Munis Muhammad Farooq Hussain, Haroon Urooj, Arif Samiah, Saqib Saddam, Zaman Wajid, Khan Ali Raza, Shi Jianxin, Che Shengquan, Liu Qunlu
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Biology (Basel). 2020 Dec 15;9(12):469. doi: 10.3390/biology9120469.
Rapidly increasing industry has resulted in greater discharge of hazardous chemicals in the soil. In the current study, soil samples were collected from Nanjing mine (32°09'19.29″ N 118°56'57.04″ E) and subjected to heavy metal analysis and microbe isolation. A total of 460 fungi were isolated, and five of these were yeast strains. Most of the strains exhibited tolerance to one metal. Five multimetal tolerant strains were selected and identified as , , , and . Isolated strains were grown in high concentrations of cadmium (Cd), chromium (Cr) and lead (Pb), for induced-tolerance training. The tolerance index (TI) revealed the highest Cd tolerance of novel strain at 5500 ppm (TI: 0.2). also displayed resistance at 4000 ppm against Cr (TI: 0.32) and Pb (TI: 0.32). In contrast, tolerance training for was not that successful. also displayed the highest bioaccumulation capacity for Cd (25.23 mg/g), Cu (21.63 mg/g), and Pb (20.63 mg/g) at 200 ppm. Scanning electron microscopy (SEM) explored the morphological changes in the mycelia of stressed fungi. Results of this study describe this delicate approach to be species and metal dependent and suggest a potential utilization of this fungal strain for the bioremediation of contaminated soils.
快速发展的工业导致土壤中有害化学物质的排放量增加。在本研究中，从南京矿（北纬32°09'19.29″，东经118°56'57.04″）采集土壤样本，进行重金属分析和微生物分离。共分离出460株真菌，其中5株为酵母菌株。大多数菌株对一种金属具有耐受性。选择了5株耐多金属菌株，分别鉴定为 、 、 、 和 。将分离出的菌株在高浓度的镉（Cd）、铬（Cr）和铅（Pb）中培养，进行诱导耐受性训练。耐受性指数（TI）显示，新的 菌株在5500 ppm时对镉的耐受性最高（TI：0.2）。 在4000 ppm时对铬（TI：0.32）和铅（TI：0.32）也表现出抗性。相比之下， 菌株的耐受性训练不太成功。 在200 ppm时对镉（25.23 mg/g）、铜（21.63 mg/g）和铅（20.63 mg/g）也表现出最高的生物积累能力。扫描电子显微镜（SEM）研究了受胁迫真菌菌丝体的形态变化。本研究结果表明，这种精细的方法具有物种和金属依赖性，并表明这种真菌菌株在污染土壤生物修复中的潜在应用价值。
