• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

低强度磁场刺激对大鼠轻度脊髓挫伤损伤后钙介导细胞毒性的影响

Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats.

作者信息

Bhattacharyya Supti, Sahu Shivani, Kaur Sajeev, Jain Suman

机构信息

Faculty of Biological Sciences, University of Leeds, Woodhouse, Leeds, UK.

Institute of Microbial Technology, Chandigarh, India.

出版信息

Ann Neurosci. 2020 Apr;27(2):49-56. doi: 10.1177/0972753120950072. Epub 2020 Oct 7.

DOI:10.1177/0972753120950072
PMID:33335356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7724432/
Abstract

BACKGROUND

Magnetic field (MF) stimulation has the potential to reduce secondary damage and promote functional recovery after neural tissue injury. The study aimed to observe the effect of very low intensity (17.96µT) MF on general body condition, secondary damage, pain status, and locomotion.

METHODS

We exposed rats to MF (2 h/day × 3 weeks) after 6.25 mm contusion spinal injury. Locomotor behavior was evaluated by BBB score, pain assessment was done by recording threshold for tail flick, expression of voltage-gated calcium channels and extent of secondary damage in the spinal cord was assessed by immunofluorescence and Cresyl violet staining, respectively.

RESULTS

A significant ( ≤ .001) improvement in bladder function as well as BBB score was observed after MF exposure in comparison with sham and SCI over the observation period of 3 weeks. SCI group showed an increase in the threshold for vocalization after discharge, which decreased following MF exposure. Cresyl violet staining showed significantly higher tissue sparing (73%) at the epicenter after MF exposure when compared to SCI group. This was accompanied with a significant decrease in calcium channel expression in MF group as compared to SCI.

CONCLUSION

The results suggest facilitation of sensory-motor recovery after MF exposure, which could be due to attenuation of secondary damage and calcium-mediated excitotoxicity in a mild contusion rat model of SCI.

摘要

背景

磁场（MF）刺激具有减少神经组织损伤后继发性损伤并促进功能恢复的潜力。本研究旨在观察极低强度（17.96µT）MF对大鼠整体身体状况、继发性损伤、疼痛状态和运动能力的影响。

方法

在大鼠脊髓受到6.25mm挫伤后，将其暴露于MF（每天2小时×3周）。通过BBB评分评估运动行为，通过记录甩尾阈值进行疼痛评估，分别通过免疫荧光和甲酚紫染色评估脊髓中电压门控钙通道的表达和继发性损伤程度。

结果

与假手术组和脊髓损伤组相比，在3周的观察期内，暴露于MF后的大鼠膀胱功能以及BBB评分有显著（≤.001）改善。脊髓损伤组在放电后发声阈值增加，而在暴露于MF后降低。与脊髓损伤组相比，甲酚紫染色显示暴露于MF后震中处的组织保留率显著更高（73%）。与脊髓损伤组相比，MF组钙通道表达显著降低。

结论

结果表明，在轻度挫伤性脊髓损伤大鼠模型中，暴露于MF后感觉运动功能恢复得到促进，这可能是由于继发性损伤和钙介导的兴奋性毒性减弱所致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/b413172157fc/10.1177_0972753120950072-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/047fd20dd049/10.1177_0972753120950072-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/96001589e79c/10.1177_0972753120950072-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/2e62ca9d68ab/10.1177_0972753120950072-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/5c52c5025fbf/10.1177_0972753120950072-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/1433a82e61fd/10.1177_0972753120950072-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/78f60b5dfc21/10.1177_0972753120950072-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/b413172157fc/10.1177_0972753120950072-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/047fd20dd049/10.1177_0972753120950072-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/96001589e79c/10.1177_0972753120950072-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/2e62ca9d68ab/10.1177_0972753120950072-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/5c52c5025fbf/10.1177_0972753120950072-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/1433a82e61fd/10.1177_0972753120950072-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/78f60b5dfc21/10.1177_0972753120950072-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a1/7724432/b413172157fc/10.1177_0972753120950072-fig7.jpg

相似文献

1
Effect of Low Intensity Magnetic Field Stimulation on Calcium-Mediated Cytotoxicity After Mild Spinal Cord Contusion Injury in Rats.低强度磁场刺激对大鼠轻度脊髓挫伤损伤后钙介导细胞毒性的影响
Ann Neurosci. 2020 Apr;27(2):49-56. doi: 10.1177/0972753120950072. Epub 2020 Oct 7.
2
Exposure to ELF- magnetic field promotes restoration of sensori-motor functions in adult rats with hemisection of thoracic spinal cord.暴露于极低频磁场可促进胸段脊髓半横断成年大鼠感觉运动功能的恢复。
Electromagn Biol Med. 2012 Sep;31(3):180-94. doi: 10.3109/15368378.2012.695706.
3
Low-energy extracorporeal shock wave therapy promotes vascular endothelial growth factor expression and improves locomotor recovery after spinal cord injury.低能量体外冲击波疗法可促进血管内皮生长因子表达，并改善脊髓损伤后的运动功能恢复。
J Neurosurg. 2014 Dec;121(6):1514-25. doi: 10.3171/2014.8.JNS132562. Epub 2014 Oct 3.
4
Extremely low frequency magnetic field protects injured spinal cord from the microglia- and iron-induced tissue damage.极低频磁场可保护受损脊髓免受小胶质细胞和铁诱导的组织损伤。
Electromagn Biol Med. 2017;36(4):330-340. doi: 10.1080/15368378.2017.1389750. Epub 2017 Nov 15.
5
The immunomodulator decoy receptor 3 improves locomotor functional recovery after spinal cord injury.免疫调节剂诱饵受体3可改善脊髓损伤后的运动功能恢复。
J Neuroinflammation. 2016 Jun 17;13(1):154. doi: 10.1186/s12974-016-0623-6.
6
Upregulation of calcium channel alpha-2-delta-1 subunit in dorsal horn contributes to spinal cord injury-induced tactile allodynia.钙通道 α2-δ1 亚基在背角的上调导致脊髓损伤引起的触觉过敏。
Spine J. 2018 Jun;18(6):1062-1069. doi: 10.1016/j.spinee.2018.01.010. Epub 2018 Jan 31.
7
A brief period of moderate noxious stimulation induces hemorrhage and impairs locomotor recovery after spinal cord injury.短时间的适度有害刺激会导致出血，并损害脊髓损伤后的运动恢复。
Physiol Behav. 2019 Dec 1;212:112695. doi: 10.1016/j.physbeh.2019.112695. Epub 2019 Oct 21.
8
Low-energy extracorporeal shock wave therapy for promotion of vascular endothelial growth factor expression and angiogenesis and improvement of locomotor and sensory functions after spinal cord injury.低能量体外冲击波疗法促进脊髓损伤后血管内皮生长因子表达和血管生成以及改善运动和感觉功能
J Neurosurg Spine. 2016 Dec;25(6):745-755. doi: 10.3171/2016.4.SPINE15923. Epub 2016 Jul 1.
9
A combinatorial approach to modulate microenvironment toward regeneration and repair after spinal cord injury in rats.一种组合方法可调节大鼠脊髓损伤后的微环境以促进再生和修复。
Neurosci Lett. 2021 Jan 10;741:135500. doi: 10.1016/j.neulet.2020.135500. Epub 2020 Nov 14.
10
Effect of extremely low frequency magnetic field in prevention of spinal cord injury-induced osteoporosis.极低频磁场在预防脊髓损伤所致骨质疏松中的作用。
J Rehabil Res Dev. 2013;50(1):17-30. doi: 10.1682/jrrd.2011.12.0248.

引用本文的文献

1
Cortical intermittent theta burst stimulation on gait pathomechanics and urinary tract dysfunction in incomplete spinal cord injury patients: Protocol for a randomized controlled trial.皮质间歇性θ波爆发刺激对不完全性脊髓损伤患者步态病理力学和泌尿系统功能障碍的影响：一项随机对照试验方案
MethodsX. 2024 Jun 25;13:102826. doi: 10.1016/j.mex.2024.102826. eCollection 2024 Dec.
2
Delivery of TGFβ3 from Magnetically Responsive Coaxial Fibers Reduces Spinal Cord Astrocyte Reactivity In Vitro.磁响应同轴纤维递送 TGFβ3 减少体外脊髓星形胶质细胞反应性。
Adv Biol (Weinh). 2024 Oct;8(10):e2300531. doi: 10.1002/adbi.202300531. Epub 2024 Jun 27.
3

本文引用的文献

1
Traumatic Spinal Cord Injury: An Overview of Pathophysiology, Models and Acute Injury Mechanisms.创伤性脊髓损伤：病理生理学、模型及急性损伤机制概述
Front Neurol. 2019 Mar 22;10:282. doi: 10.3389/fneur.2019.00282. eCollection 2019.
2
Low‑frequency pulsed electromagnetic field promotes functional recovery, reduces inflammation and oxidative stress, and enhances HSP70 expression following spinal cord injury.低频脉冲电磁场促进脊髓损伤后的功能恢复，减轻炎症和氧化应激，增加 HSP70 表达。
Mol Med Rep. 2019 Mar;19(3):1687-1693. doi: 10.3892/mmr.2019.9820. Epub 2019 Jan 4.
3
Upregulation of calcium channel alpha-2-delta-1 subunit in dorsal horn contributes to spinal cord injury-induced tactile allodynia.
Neurotrauma-From Injury to Repair: Clinical Perspectives, Cellular Mechanisms and Promoting Regeneration of the Injured Brain and Spinal Cord.
神经创伤——从损伤到修复：临床视角、细胞机制与促进损伤脑和脊髓的再生
Biomedicines. 2024 Mar 13;12(3):643. doi: 10.3390/biomedicines12030643.
4
Omega-conotoxin MVIIA reduces neuropathic pain after spinal cord injury by inhibiting N-type voltage-dependent calcium channels on spinal dorsal horn.ω-芋螺毒素MVIIA通过抑制脊髓背角的N型电压依赖性钙通道来减轻脊髓损伤后的神经性疼痛。
Front Neurosci. 2024 Feb 26;18:1366829. doi: 10.3389/fnins.2024.1366829. eCollection 2024.
5
Spinal cord injury: molecular mechanisms and therapeutic interventions.脊髓损伤：分子机制与治疗干预。
Signal Transduct Target Ther. 2023 Jun 26;8(1):245. doi: 10.1038/s41392-023-01477-6.
6
Electromagnetic Field Stimulation Attenuates Phasic Nociception after Complete Spinal Cord Injury in Rats.电磁场刺激减轻大鼠完全性脊髓损伤后的相性伤害感受。
Brain Sci. 2021 Oct 28;11(11):1431. doi: 10.3390/brainsci11111431.
钙通道 α2-δ1 亚基在背角的上调导致脊髓损伤引起的触觉过敏。
Spine J. 2018 Jun;18(6):1062-1069. doi: 10.1016/j.spinee.2018.01.010. Epub 2018 Jan 31.
4
Extremely low frequency magnetic field protects injured spinal cord from the microglia- and iron-induced tissue damage.极低频磁场可保护受损脊髓免受小胶质细胞和铁诱导的组织损伤。
Electromagn Biol Med. 2017;36(4):330-340. doi: 10.1080/15368378.2017.1389750. Epub 2017 Nov 15.
5
Extremely Low Frequency Electromagnetic Fields Facilitate Vesicle Endocytosis by Increasing Presynaptic Calcium Channel Expression at a Central Synapse.极低频电磁场通过增加中枢突触前钙通道表达促进囊泡内吞作用。
Sci Rep. 2016 Feb 18;6:21774. doi: 10.1038/srep21774.
6
Cellular and molecular changes to cortical neurons following low intensity repetitive magnetic stimulation at different frequencies.不同频率的低强度重复磁刺激对皮质神经元的细胞和分子变化。
Brain Stimul. 2015 Jan-Feb;8(1):114-23. doi: 10.1016/j.brs.2014.09.012. Epub 2014 Oct 16.
7
A consistent, quantifiable, and graded rat lumbosacral spinal cord injury model.一种一致、可量化且分级的大鼠腰骶部脊髓损伤模型。
J Neurotrauma. 2015 Jun 15;32(12):875-92. doi: 10.1089/neu.2013.3321. Epub 2015 Mar 12.
8
Tissue sparing, behavioral recovery, supraspinal axonal sparing/regeneration following sub-acute glial transplantation in a model of spinal cord contusion.亚急性胶质细胞移植治疗脊髓挫伤模型中的组织保护、行为恢复、脊髓以上轴突保留/再生。
BMC Neurosci. 2013 Sep 27;14:106. doi: 10.1186/1471-2202-14-106.
9
Iron oxide nanoparticles and magnetic field exposure promote functional recovery by attenuating free radical-induced damage in rats with spinal cord transection.氧化铁纳米颗粒和磁场暴露通过减轻自由基诱导的损伤促进脊髓横断大鼠的功能恢复。
Int J Nanomedicine. 2013;8:2259-72. doi: 10.2147/IJN.S44238. Epub 2013 Jun 21.
10
Exposure to extremely low-frequency magnetic field restores spinal cord injury-induced tonic pain and its related neurotransmitter concentration in the brain.暴露于极低频磁场可恢复脊髓损伤诱导的紧张性疼痛及其在大脑中的相关神经递质浓度。
Electromagn Biol Med. 2013 Dec;32(4):471-83. doi: 10.3109/15368378.2012.743907. Epub 2013 May 8.