Hamilton Matthew G, Lind Curtis E, Barman Benoy K, Velasco Ravelina R, Danting Ma Jodecel C, Benzie John A H
WorldFish, Penang, Malaysia.
Department of Aquatic Resources, Ecology and Management, College of Fisheries, Central Luzon State University, Muñoz, Philippines.
Front Genet. 2020 Dec 1;11:594722. doi: 10.3389/fgene.2020.594722. eCollection 2020.
Nile tilapia () is among the most important finfish in aquaculture, particularly in Asia. Numerous genetically improved strains of Nile tilapia have been developed and disseminated through formal and informal channels to hatcheries, many of which operate at a relatively small scale in developing countries. The primary objective of this study was to assess the extent to which molecular genetic tools can identify different and interrelated strains of Nile tilapia in Bangladesh and the Philippines, two globally significant producers. A tool was developed using a low-density panel of single-nucleotide polymorphisms (SNPs), genotyping-by-sequencing and discriminant analysis of principal components (DAPC). When applied to 2,057 samples from 205 hatcheries in Bangladesh and the Philippines, for hatcheries where the hatchery-identified strain was one of the sampled core populations used to develop the tool, hatchery-identified and DAPC-assigned hatchery-level strains were in agreement in 74.1% of cases in Bangladesh and 80.6% of cases in the Philippines. The dominant hatchery-identified and DAPC-assigned strains were GIFT, in Bangladesh, and GET-ExCEL-a composite strain partially derived from GIFT-in the Philippines.
尼罗罗非鱼（）是水产养殖中最重要的硬骨鱼之一，在亚洲尤其如此。已经通过正式和非正式渠道开发并向孵化场传播了许多基因改良的尼罗罗非鱼品系，其中许多孵化场在发展中国家规模相对较小。本研究的主要目的是评估分子遗传学工具在多大程度上能够识别孟加拉国和菲律宾这两个全球重要生产国的尼罗罗非鱼不同且相互关联的品系。利用低密度单核苷酸多态性（SNP）面板、简化基因组测序和主成分判别分析（DAPC）开发了一种工具。当将该工具应用于来自孟加拉国和菲律宾205个孵化场的2057个样本时，对于孵化场鉴定的品系是用于开发该工具的采样核心群体之一的孵化场，在孟加拉国，孵化场鉴定的和DAPC分配的孵化场水平品系在74.1%的情况下一致，在菲律宾为80.6%。在孟加拉国，孵化场鉴定的和DAPC分配的主要品系是吉富品系（GIFT），在菲律宾是GET-ExCEL——一种部分源自吉富品系的复合品系。
