Lind C E, Kilian A, Benzie J A H
WorldFish, Jalan Batu Maung, 11960, Bayan Lepas, Penang, Malaysia.
Diversity Arrays Technology Pty Ltd (DArT P/L), University of Canberra, Building 3, Bruce, ACT, 2617, Australia.
Anim Genet. 2017 Jun;48(3):362-364. doi: 10.1111/age.12536. Epub 2017 Jan 17.
The development of genomic markers is described for Nile tilapia, Oreochromis niloticus, using the Diversity Arrays Technology (DArT) genotype-by-sequencing platform. A total of 13 215 single nucleotide polymorphism (SNP) markers and 12 490 silicoDArT (dominant) markers were identified from broodstock of two selective breeding programs [Genetically Improved Farmed Tilapia (GIFT) strain from Malaysia and the Abbassa strain from Egypt]. Over 10 000 SNPs were polymorphic in either strain, and 2985 and 3087 showed strain-specific polymorphisms for the GIFT and Abbassa strains respectively. We demonstrate the potential utility of these markers for rapid genomic screening and use in breeding programs.
利用多样性阵列技术（DArT）基因分型测序平台，描述了尼罗罗非鱼（Oreochromis niloticus）基因组标记的开发情况。从两个选择性育种计划的亲鱼群体[马来西亚的遗传改良养殖罗非鱼（GIFT）品系和埃及的阿巴萨品系]中，共鉴定出13215个单核苷酸多态性（SNP）标记和12490个硅DArT（显性）标记。超过10000个SNP在任一品系中具有多态性，其中2985个和3087个分别显示出GIFT品系和阿巴萨品系的品系特异性多态性。我们展示了这些标记在快速基因组筛选和育种计划中的潜在用途。
