Maity Debabrata
Department of Chemistry, New York University, New York, NY 10003, USA.
Beilstein J Org Chem. 2020 Dec 3;16:2971-2982. doi: 10.3762/bjoc.16.247. eCollection 2020.
To understand the molecular interactions, present in living organisms and their environments, chemists are trying to create novel chemical tools. In this regard, peptide-based fluorescence techniques have attracted immense interest. Synthetic peptide-based fluorescent probes are advantageous over protein-based sensors, since they are synthetically accessible, more stable, and can be easily modified in a site-specific manner for selective biological applications. Peptide receptors labeled with environmentally sensitive/FRET fluorophores have allowed direct detection/monitoring of biomolecules in aqueous media and in live cells. In this review, key peptide-based approaches for different biological applications are presented.
为了理解存在于生物体及其环境中的分子相互作用，化学家们正在尝试创造新型化学工具。在这方面，基于肽的荧光技术引起了极大的兴趣。基于合成肽的荧光探针比基于蛋白质的传感器更具优势，因为它们可以通过合成获得，更稳定，并且可以以位点特异性方式轻松修饰以用于选择性生物应用。用对环境敏感的/荧光共振能量转移（FRET）荧光团标记的肽受体能够直接检测/监测水性介质和活细胞中的生物分子。在这篇综述中，介绍了用于不同生物应用的基于肽的关键方法。
