Kiesewalter Heiko T, Lozano-Andrade Carlos N, Strube Mikael L, Kovács Ákos T
Bacterial Interactions and Evolution Group, DTU Bioengineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
Bacterial Ecophysiology and Biotechnology Group, DTU Bioengineering, Technical University of Denmark, Kgs. Lyngby, Denmark.
Beilstein J Org Chem. 2020 Dec 4;16:2983-2998. doi: 10.3762/bjoc.16.248. eCollection 2020.
Secondary metabolites provide with increased competitiveness towards other microorganisms. In particular, nonribosomal peptides (NRPs) have an enormous antimicrobial potential by causing cell lysis, perforation of fungal membranes, enzyme inhibition, or disruption of bacterial protein synthesis. This knowledge was primarily acquired in vitro when was competing with other microbial monocultures. However, our understanding of the true ecological role of these small molecules is limited. In this study, we have established soil-derived semisynthetic mock communities containing 13 main genera and supplemented them with P5_B1 WT, the NRP-deficient strain , or single-NRP mutants incapable of producing surfactin, plipastatin, or bacillaene. Through 16S amplicon sequencing, it was revealed that the invasion of NRP-producing strains had no major impact on the bacterial communities. Still, the abundance of the two genera and was reduced. Interestingly, this effect was diminished in communities supplemented with the NRP-deficient strain. Growth profiling of M5 exposed to either spent media of the strains or pure surfactin indicated the sensitivity of this strain towards the biosurfactant surfactin. Our study provides a more in-depth insight into the influence of NRPs on semisynthetic bacterial communities and helps to understand their ecological role.
次级代谢产物使微生物对其他微生物具有更强的竞争力。特别是，非核糖体肽（NRPs）具有巨大的抗菌潜力，可导致细胞裂解、真菌细胞膜穿孔、酶抑制或破坏细菌蛋白质合成。这些知识主要是在体外与其他微生物单培养物竞争时获得的。然而，我们对这些小分子真正生态作用的理解是有限的。在本研究中，我们建立了包含13个主要属的土壤来源的半合成模拟群落，并用P5_B1 WT、NRP缺陷菌株或无法产生表面活性素、脂肽素或杆菌烯的单NRP突变体对其进行补充。通过16S扩增子测序发现，产生NRP的菌株的入侵对细菌群落没有重大影响。不过，两个属的丰度降低了。有趣的是，在添加了NRP缺陷菌株的群落中，这种影响减弱了。暴露于菌株的陈旧培养基或纯表面活性素的M5的生长分析表明该菌株对生物表面活性剂表面活性素敏感。我们的研究更深入地洞察了芽孢杆菌属NRPs对半合成细菌群落的影响，并有助于理解它们的生态作用。
