Ye Limin, Zhu Liyi, Wang Jinglin, Li Fei
Department of Gastroenterology, People's Hospital of Guizhou Province, No. 83, Zhongshan East Road, Nanming District, Guiyang, Guizhou 550002, China.
Open Med (Wars). 2020 Jul 13;15(1):663-671. doi: 10.1515/med-2020-0137. eCollection 2020.
Hepatoma is a serious liver cancer with high morbidity and mortality. Eldecalcitol (ED-71), a vitamin D analog, is extensively used as anti-cancer agent . Hepatocellular carcinoma cell, SMMC-7721 cell lines were used in this study. Transwell assay, cell apoptosis and cell cycle detection assays were investigated after treatment with ED-71 and phosphate buffered saline (PBS) as control. Sizes of tumors were measured after ED-71 treatment in a mouse model. E-cadherin and Akt gene expressions were detected by real-time PCR (RT-PCR). The results showed that cell invasion and migration were decreased markedly after ED-71 treatment compared to control group. Cell cycle detection showed that the G2 stage was 13.18% and total S-stage was 41.16% in the ED-71 group and G2 stage: 22.88%, total S-stage: 27.34% in the control group. Cell apoptosis rate was promoted in the ED-71 group. Size of the tumors reduced more after the ED-71 treatment than the PBS treatment in mice. ED-71 markedly inhibited the expression of Akt and E-cadherin, either detected by immunohistochemistry or RT-PCR. ED-71 treatment can inhibit the hepatoma agent proliferation by increasing the E-cadherin expression and decreasing Akt expression. Therefore, these findings provide novel evidence that ED-71 can be used as an anti-hepatoma agent.
肝癌是一种发病率和死亡率都很高的严重肝癌。 eldecalcitol（ED-71），一种维生素D类似物，被广泛用作抗癌剂。本研究使用了肝癌细胞SMMC-7721细胞系。在用ED-71和作为对照的磷酸盐缓冲盐水（PBS）处理后，研究了Transwell实验、细胞凋亡和细胞周期检测实验。在小鼠模型中用ED-71处理后测量肿瘤大小。通过实时PCR（RT-PCR）检测E-钙黏蛋白和Akt基因表达。结果显示，与对照组相比，ED-71处理后细胞侵袭和迁移明显减少。细胞周期检测显示，ED-71组的G2期为13.18%，总S期为41.16%，对照组的G2期为22.88%，总S期为27.34%。ED-71组的细胞凋亡率升高。在小鼠中，ED-71处理后肿瘤大小比PBS处理后缩小得更多。通过免疫组织化学或RT-PCR检测，ED-71均显著抑制Akt和E-钙黏蛋白的表达。ED-71处理可通过增加E-钙黏蛋白表达和降低Akt表达来抑制肝癌细胞增殖。因此，这些发现提供了新的证据，表明ED-71可作为一种抗肝癌药物。
