raf-1 激酶抑制剂蛋白在口腔鳞状细胞癌中的临床意义及其在细胞转移中的作用。
Clinical significance of raf-1 kinase inhibitor protein in oral squamous cell carcinoma and its role in cell metastasis.
机构信息
Department of Oral and Maxillofacial Surgery, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12194-12199. doi: 10.26355/eurrev_202012_24009.
OBJECTIVE
To detect the expression pattern of raf-1 kinase inhibitor protein (RKIP) in oral squamous cell carcinoma (OSCC) samples and to explore its clinical significance in OSCC metastasis.
PATIENTS AND METHODS
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot assay were conducted to detect the expression of RKIP in OSCC tissues and cells. The relationship between RKIP expression and OSCC clinicopathological characteristics was statistically analyzed. Transwell assay, wound healing assay, and Western blot were used to detect the influence of RKIP on the metastasis ability of OSCC cells.
RESULTS
RKIP was significantly downregulated in OSCC samples. Low expression of RKIP predicted high incidence of metastasis in OSCC patients. In vitro experiments demonstrated that overexpression of RKIP could significantly inhibit invasion and migration abilities of OSCC cells.
CONCLUSIONS
RKIP was a novel factor involved in OSCC progression, which was a potential biomarker and therapeutic target for the patients.
目的
检测 raf-1 激酶抑制剂蛋白（RKIP）在口腔鳞状细胞癌（OSCC）样本中的表达模式，并探讨其在 OSCC 转移中的临床意义。
方法
采用实时定量聚合酶链反应（qRT-PCR）和 Western blot 检测 RKIP 在 OSCC 组织和细胞中的表达。统计分析 RKIP 表达与 OSCC 临床病理特征的关系。Transwell 检测、划痕愈合检测和 Western blot 用于检测 RKIP 对 OSCC 细胞转移能力的影响。
结果
RKIP 在 OSCC 样本中显著下调。RKIP 低表达预示着 OSCC 患者转移发生率高。体外实验表明，RKIP 的过表达可显著抑制 OSCC 细胞的侵袭和迁移能力。
结论
RKIP 是参与 OSCC 进展的一个新的因素，是患者的一个潜在的生物标志物和治疗靶点。
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