Department of Medical Ontology, First Affiliated Hospital of Soochow University, No. 188, Shi Zi Rd, Soochow 215006, People's Republic of China.
Pathol Oncol Res. 2013 Jan;19(1):19-26. doi: 10.1007/s12253-012-9552-6. Epub 2012 Jun 13.
The purpose of this study was to investigate the expression of Raf kinase inhibitor protein (RKIP) and epithelial cadherin (E-cadherin) in lung squamous cell carcinoma tissue and its correlation with the clinical pathology of lung squamous cell carcinoma. RKIP and E-cadherin mRNA (by RT-PCR) and protein (by western blotting) levels were monitored in carcinoma tissues and surrounding normal tissues from 86 lung squamous cell carcinoma cases, and their positive rates were calculated. The rates of positive RKIP and E-cadherin mRNA expression were significantly lower in lung squamous cell carcinoma than in the surrounding normal tissues (P < 0.05). The positive expression rates were significantly lower in those with lymph node metastasis than in those without (P < 0.05). The lower the degree of tumor differentiation, the lower the E-cadherin mRNA positive expression rate (P < 0.05). The rates of positive RKIP and E-cadherin mRNA expression were significantly lower in patients at advanced (III, IV) stages than in patients at early (I, II) stages (p < 0.05); this rate, however, was independent of gender, age, and tumor size (P > 0.05). The protein levels of RKIP and E-cadherin were significantly lower in lung squamous cell carcinoma than in the surrounding normal tissues (P < 0.05). The levels were significantly lower in patients with lymph node metastasis than in those without it (P < 0.05). The lower the degree of tumor differentiation, the lower the protein level of E-cadherin (P < 0.05). Both RKIP and E-cadherin are tumor suppressors, their low expression levels may be associated with initiation, invasion and/or metastasis, as well as with the inhibition of lung squamous cell carcinoma differentiation.
本研究旨在探讨 Raf 激酶抑制剂蛋白（RKIP）和上皮钙黏蛋白（E-cadherin）在肺鳞癌组织中的表达及其与肺鳞癌临床病理的关系。监测 86 例肺鳞癌患者癌组织及癌旁正常组织中 RKIP 和 E-cadherin mRNA（RT-PCR）和蛋白（western blot）的水平，并计算其阳性率。肺鳞癌组织中 RKIP 和 E-cadherin mRNA 的阳性率明显低于癌旁正常组织（P<0.05）；有淋巴结转移者明显低于无淋巴结转移者（P<0.05）；肿瘤分化程度越低，E-cadherin mRNA 阳性表达率越低（P<0.05）。Ⅲ、Ⅳ期患者的 RKIP 和 E-cadherin mRNA 阳性表达率明显低于Ⅰ、Ⅱ期患者（P<0.05）；但与性别、年龄、肿瘤大小无关（P>0.05）。肺鳞癌组织中 RKIP 和 E-cadherin 蛋白的表达水平明显低于癌旁正常组织（P<0.05）；有淋巴结转移者明显低于无淋巴结转移者（P<0.05）；肿瘤分化程度越低，E-cadherin 蛋白水平越低（P<0.05）。RKIP 和 E-cadherin 均为肿瘤抑制因子，其低表达可能与肺鳞癌的发生、浸润和/或转移以及分化抑制有关。
