Biosafety Division, National Institute of Agricultural Sciences, Jeonju, South Korea.
R&D Coordination Division, Rural Development Administration, Jeonju, South Korea.
J Sci Food Agric. 2021 Apr;101(6):2601-2613. doi: 10.1002/jsfa.11028. Epub 2021 Jan 18.
PfFAD3 transgenic soybean expressing omega-3 fatty acid desaturase 3 of Physaria produces increased level of α-linolenic acid in seed. Composition data of non-transgenic conventional varieties is important in the safety assessment of the genetically-modified (GM) crops in the context of the natural variation.
The natural variation was characterized in seed composition of 13 Korean soybean varieties grown in three locations in South Korea for 2 years. Univariate analysis of combined data showed significant differences by variety and cultivation environment for proximates, minerals, anti-nutrients, and fatty acids. Percent variability analysis demonstrated that genotype, environment and the interaction of environment with genotype contributed to soybean seed compositions. Principal component analysis and orthogonal projections to latent structure discriminant analysis indicated that significant variance in compositions was attributable to location and cultivation year. The composition of three PfFAD3 soybean lines for proximates, minerals, anti-nutrients, and fatty acids was compared to a non-transgenic commercial comparator (Kwangankong, KA), and three non-transgenic commercial varieties grown at two sites in South Korea. Only linoleic and linolenic acids significantly differed in PfFAD3-1 lines compared to KA, which were expected changes by the introduction of the PfFAD3-1 trait in KA.
Genotype, environment, and the interaction of environment with genotype contributed to compositional variability in soybean. PfFAD3-1 soybean is equivalent to the conventional varieties with respect to these components. © 2020 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
表达来自 Physaria 的ω-3 脂肪酸去饱和酶 3 的 PfFAD3 转基因大豆在种子中产生了 α-亚麻酸水平的增加。非转基因常规品种的组成数据对于在自然变异背景下对转基因作物的安全性评估很重要。
对在韩国三个地点种植的 13 个韩国大豆品种的种子组成进行了 2 年的自然变异特征分析。综合数据的单变量分析表明，品种和栽培环境对粗蛋白、矿物质、抗营养因子和脂肪酸有显著差异。百分比变异性分析表明，基因型、环境以及环境与基因型的相互作用对大豆种子组成有贡献。主成分分析和正交投影到潜在结构判别分析表明，组成的显著差异归因于地点和栽培年份。将 PfFAD3 大豆品系的组成与非转基因商业对照（光冈空，KA）和在韩国两个地点种植的三个非转基因商业品种进行了比较。只有亚油酸和亚麻酸在 PfFAD3-1 品系与 KA 之间存在显著差异，这是通过在 KA 中引入 PfFAD3-1 性状而预期的变化。
基因型、环境以及环境与基因型的相互作用导致大豆组成的可变性。PfFAD3-1 大豆在这些成分方面与常规品种相当。© 2020 作者。John Wiley & Sons Ltd 代表化学工业协会出版的《食品科学杂志》。
